Core Practical 4

Question 1

how do low temperatures prevent reactions or slow them down

Answer 1

• molecules will move slower
• lower frequency of successful collisions between substrate and active site
• less frequent enzyme-substrate complex formation
• substrate and enzyme collide with less energy so less likely to bond

Question 2

how do high temperatures prevent reactions or slow them down

Answer 2

• molecules will move faster
• higher frequency of successful collisions between substrate and active site
• more frequent enzyme-substrate complex formation
• substrate and enzyme collide with more energy so more likely to bond

Question 3

what happens when the temperature continue to increase

Answer 3

• hydrogen bonds will break
• the tertiary structure changes
• which damages the active site and prevents it from binding to substrate
• which will cause denaturation

Question 4

how does enzyme concentration affect the rate of reaction

Answer 4

-the higher the number of active sites for the substrate to collide
- the substrate will be a limiting factor if there is not enough of substrate the reaction will stop

Question 5

how does substrate concentration affect the rate of reaction

Answer 5

• as substrate conc. increases the collision between substrate and active site increases
• the conc, of substrate will decrease overtime