control of gene expression Flashcards
substitution (types of mutations) are…
one or more baes are substituted in the base sequence of DNA, may change to amino acid sequence, may not due to degenerate nature of the genetic code (silent)
silent/ neutral mutations (types of mutations) are…
mutation occurs in non-coding region of DNA, change in tertiary structure of the protein has no major effect on the organism
duplication (types of mutations) are…
one or more bases are repeated and therefore produced a frameshift, same effect as an insertion
inversion (types of mutations) are…
a group of bases become separated from the DNA base sequence + re-join at the same position but in reverse order
changes ion the amino acid sequence + therefore primary + tertiary structure of protein
insertion/deletion (types of mutations) are…
insertion- one or more nucleotide pairs are inserted into the sequence, frameshift to the right
deletion- one or more nucleotide pairs are deleted from the sequence, frameshift to the left
translocation (types of mutations) is…
group of bases become separated from DNA base sequences on one chromosome + are inserted into the DNA sequence on another chromosome
non-sense mutation is…
premature stop codon
mis-sense mutation is…
different amino acid coded for
causes of mutation are…
spontaneous error during DNA replication
chemical mutagens- benzene, alcohol, asbestos, tobacco
ionising radiation- UV, X-ray, alpha, beta
what are stem cells?…
unspecialised cells that continually divide and become specialised
differentiation= process when stem cells become specialised
types= totipotent, pluripotent, multipotent and unipotent
totipotent stem cells can…
differentiate into any type of body cell, and any cell needed for development
during development, can translate part of their DNA, resulting in specialisation
only occur for a limited time in early mamaliam embryos
pluripotent stem cells are…
found in embryos
can divide in unlimited numbers and differentiate into almost any type of body cell
used in treating human disorders
issues= can keep dividing causing a tumour, ethical problem, is it right for humans to be cloned
multipotent and pluripotent stem cells are…
found in mature mammals
multipotent= differentiate into a limited number of cells like bone marrow
unipotent= can only differentiate into one type of cell
iPS cells are…
created from adult unipotent cells (somatic)
treated with transcription factors to switch on genes that induced pluripotent (undifferentiated)
advantages of iPS cells are…
don’t cause embryonic destruction
self-renewal property, so can divide indefinitely to give a limitless supply
used in medical treatment instead of embryonic stem cells
controlling of transcription is when…
transcription of target genes can be either inhibited or stimulated when specific transcription factors move from the cytoplasm to the nucleus
controlling of gene expression (turning ‘off’ or ‘on’
transcription factors are…
molecules that move from the cytoplasm to the nucleus
bind to DNA in the promotor/operator region, initiating/inhibiting transcription and therefore translation of the protein
without transcription factors, gene is ‘turned off’, so the protein isn’t made
the role of oestrogen in initiating transcription is…
enters the cytoplasm through phospholipid bolster as it’s lipid soluble
binds to receptor site on transcription factor
changes tertiary structure, so TF is now complementary to and can bind to the promotor region of DNA initiating transcription
epigenetic control is…
epigenetic= environmental changes that cause heritable changes in gene function without changing the DNA base sequence
epigenetic control is mediated by chemical ‘tags’, which are collectively called the epigenome
some inhibit transcription= increased methylation of DNA+decreased acetylation if associated histones
increase methylation of DNA is…
methyl groups added to DNA at cytosine bases (sometimes called CpG islands)
prevents transcription factors from binding to promotor region of DNA
attracts proteins that condes de chromatin (DNA-his tone complex), so preventing transcription of RNA polymerase can’t bind
decreased acetylation of histones is…
histones become more positively charged, attracting phosphate groups on DNA as phosphate is negatively charged
causes chromatin to condense, so transcription factors + RNA polymerase can’t bind