control and inheritance of transcription regulation; RNA processing Flashcards
which are the 6 controls of eukaryotic gene expression
transcriptional control RNA processing control RNA transport and localization control translation control mRNA degradation control Protein activity control
where is the base pairs present unique pattern?
major groove
what is the protein that can switch off the transcription?
repressor protein
what is the protein that turns on the transcription ?
activator protein
what are the important factor in the mechanism of transcription regulation
when lactose is present
and
glucose is absent
how can the transcription activators changes the chromatin structure ?
Chromatin remodeling complex:
- -> allows access of transcription machinery to DNA
- -> assembles on nucleosome-free DNA
- -> allow greater access to nucleosome DNA
Histone-modifying enzyme
–> destabilize forms of chromatin and attract transcription components
what are the transcription regulator activation?
protein synthesis ligand binding covalent modification addition of subunit unmasking stimulation of nuclear entry release from membrane
what is imprinting
form of epigenetic inheritance where expression of a gene is dependent on the sex of the transmitting parent
What is RNA splicing
removes intron sequences from newly transcribed pre-mRNA
how the mechanism of Pre-mRNA splicing works?
adenine nucleotide attacks 5’ splice site
5’ splice site is cut, formation of loop
free hydroxyl group attached to 3’s splice site release of lariat structure
what principle that shows the successful capping of the mature mRNA?
presence of CBC (Cap binding complex)
Presence of EJC (Exon junction complex)
Presence of Poly-A-binding protein marks successful poly-A addition
absence of snRNP
What dose the RNA polymerase C-Terminal Domain (CTD) do?
Provides a “landing platform” for different protein involved in 5’ end capping, splicing and addition of the poly-A tail
