Connective/Adipose Tissue

Question 1

Collagen Fibers Staining

Answer 1

Stain readily with eosin and other acidic dyes

Blue: Mallory’s (Azan) stain
Light green: Masson’s trichrome stain
Red: picrosirius

Question 2

Type I collagen

Answer 2

Loose and dense connective tissue (skin, bone, tendon…)

Defect: Osteogenesis Imperfecta

Question 3

Type II collagen

Answer 3

Cartilage

Defect: Achodrogenesis

Question 4

Type III collagen

Answer 4

Forms of reticular fibers

Defect: Ehlers Danlos

Question 5

Type IV collagen

Answer 5

Basal Lamina

Question 6

Type VII collagen

Answer 6

Anchoring fibrils of skin

Question 7

Type VIII collagen

Answer 7

Endothelial cells

Question 8

Elastic fibers stains

Answer 8

Orcein (Brown/Black)
Resorcin-fuchsin
Aldehyde-fuchsin (Dark Magenta)
Weigert’s

Question 9

GAG Type: Hyaluronan

Answer 9

Umbilical cord, synovial fluid

Question 10

GAG Type: Chondroitin-4-sulfate

Answer 10

Cartilage, bone

Question 11

GAG Type: Chondroitin-6-phosphate

Answer 11

Cartilage, bone

Question 12

GAG Type: Dermatan sulfate

Answer 12

Skin, blood vessels

Question 13

GAG Type: Keratan sulfate

Answer 13

Bone, cartilage

Question 14

GAG Type: Heparan sulfate

Answer 14

Basal lamina, cell surface

Question 15

Heparin

Answer 15

Mast cell granules

Question 16

Mature (white) adipocyte staining

Answer 16

Oil Red O: Red

Osmium tetroxide: Black

Sudan Black: Black