Comprehensive Review Flashcards
Review for pre boards
Primary goal of immunolgy
Immunity
Discovered cellular theory of immunity
Metchnikoff 1883 to 1905
Prevents entry and establishment of pathogen in the body
External defence system
Bridges innate and adaptive immunity
Largest lymphocyte
Nk cells
Multicolony stimulating factor
IL 3
Alpha 1 antirtrypsin deficiency
Premature emphysema/liver dse
Most potent opsonin
CRP
Location of MHC
Chromosome 6p
Most potent APC
Dendritic cells
Massive overproduction of cytokine leading to shock , multi organ failure
Cytokine storm
Rearrangement of genes coding for heavy and light chains of antibody
Pro b cells
Synthesis of heavy chain
Pre B cell
Immunohlobulin present in mature B cells
Surface IgD and IgM
Most efficient in fixing complement
IgG3
who developed the side chain theory of anti-body?
Ehrlich
steps in PCR
Denaturation- heat at 95
annealing-cool to 52
Elongation-72
at which stage of syphilis does hard chancre be seen
Primary
confirmatory test of HIV in the Philippines
RHIVDA
what are the bands present in the confirmatory test of HIV
p24, GP 120/160, gp41
screening test for HIV
ELISA
A marker of hepatitis that shows immunity
Anti-HBs
A marker of hepatitis that shows recovery
Anti-HBe
A lifelong marker of hepatitis
IgG Anti-HBc
A marker of hepatitis that shows current or recent infection “Core Window Period”
IgM Anti-HBc
A marker of hepatitis that shows a high degree of infectivity
HBeAg
A marker of hepatitis that shows active infection
HBsAg
The most specific cells containing antigens that agglutinate with heterophile antibodies
Sheep erythrocytes
Serologic test that differentiates Heterophile anti-bodies associated with infectious mononucleosis, serum sickness or Forssman Ag
Davidsohn differential test
Largest cell in the peripheral Blood
Monocyte
MHC CLASS 1
All nucleated cells
CD8
Endogenous Ag
structure 3 alpha; 1 beta
Antigen in MHC CLASS 3
Benett Goodspeed
Gold standard for identification of lymphocyte
Flow Cytometry
Enzyme Digestion that cleaves an antibody molecule into 3 pieces = 2 Fab; 1 Fc
Papain; Rodney Porter
Enzyme Digestion that cleaves an antibody molecule into 2 pieces = 1 Fab; 1 Fc
Pepsin; Alfred Nisonoff
Variable Regions of immunoglobulins are found in what End of each chain
Amino-terminal End
Constant region of immunoglobulins are found in what end of each chain
Carboxyl-terminal end
a compound that enhances immune response, it is not immunogenic and cannot induce an antibody response
Adjuvant
Formerly used as potentiator before PEG
Sendai Virus
Medium for Hybridoma technique
Hypoxanthine-Aminopterine-Thymidine
The structure formed by the fusion of engulfed materials and enzymatic granules within a phagocytic cell is called a
Phagolysosome
IgM
Fixes Complement
Has a J Chain
Primary response antibody
Bence Jones Protein is identical to
L chains
soluble antibody combines with soluble antigen
Precipitation
Antibody + particulate antigen
Agglutination
unit of sedimentation rate of imunoglobulins
Svedberg
antigens capable of inducing immune response
immunogens
Complement system is inactivated at
56-degree celsius for 30 mins- use within 4 hours
complement deficiency that has no known associated dse
C9
most common complement deficiency
C2
Most severecomplement deficiency
C3
preferred method of screening type 1 (hypersensitivty reacton)
in vivo prick test
Most immunogenic
Bone Marrow
Least immunogenic
Cornea
plays an important role as an external defense mechanism?
Phagocytosis
Flagellar antigens are designated
H antigen
A graft from a mother to her child would be a/an
Allograft
A negative test for VDRL but positive for FTA-ABS correlate with
Early syphillis
Serologic diagnosis for cryptococcus can be made by testing what specimen/s?
CSF
In agglutination, if cells are not centrifuged long enough the results would be
False Negative
exact binding type of an antigen
Epitope
complement deficiency associated with Hereditary Angiodema
C1INH
it measures the total serum IgE
Radioimmunosorbent test (RIST)
it measures antigen specific IgE
Radioallergosorbent test (RAST)
