Component Preparation Flashcards
Collection of blood in a sterile blood container
Closed system
Collection or exposure to air through open ports that would shorten the expiration because of potential bacterial contamination
Open system
The volume of anticoagulant-preservative solutions in the primary collection bag
63-70mL (63mL for 450+/- 45mL blood drawn;
70 mL for 500+/- 50mL blood drawn)
Considered as Red blood cells low volume
300-404 mL of whole blood in an anticoagulant volume calculated for 450+/- 45mL;
333-449 mL of whole blood in an anticoagulant volume calculated for 500+/-50mL
Components discarded for low volume units
Platelets, FFP, Cryoprecipitate AHF,
Storage lesion: Increase & Decrease
Increase: Plasma K+, Plasma Hemoglobin, NH4
Decrease: Viable cells, Plasma pH, Plasma Na+, RBC ATP & 2,3 DPG
The storage limit for CPD (Citrate - phosphate-dextrose)
21 days
The storage limit for CP2D (Citrate-phosphate-2-dextrose)
21 days
The storage limit for CPDA-1 (Citrate-phosphate-dextrose-adenine)
35 days
The storage limit for AS-1, AS-3, AS-5, AS-7
42 days
RBCs prepared from blood collected in CPDA-1 should have a hematocrit level of ___
=80% HCT
An anticoagulant-preservative chemical that prevents coagulation by chelating calcium
Citrate
Storage temperature for Whole blood, RBCs, WRBCs / deglycerolized RBCs, Irradiated RBCs
1-6C
Storage temperature for Frozen RBCs
-65C
Expiration limit for Frozen RBCs with CPD / CPDA-1 additive
10 years
Storage temperature(s) for FFP, and corresponding expiration limits
= -18C : 1 yr.
= -65C : 7 yrs.
Platelets should be stored at ___ with continuous ___.
20-24C with continuous agitation
A buffered salt solution that replaces a portion of the plasma to store platelets
PAS (Platelet Additive Solution)
Storage temperature of thawed plasma and expiration limit
1-6C; 5 days from thawing
Whole blood is prepared with a ratio of __ mL anticoagulant preservative solution per ___ mL of whole blood.
14 mL anticoagulant preservative solution per 100 mL of whole blood
Components of whole blood
RBCs, WBCs, platelets, plasma proteins, and anticoagulant-preservative solution
Whole blood is administered to patients who are actively bleeding and have lost more than __% of their blood volume.
25%
Whole blood increases the hemoglobin by __g/dL, or the hematocrit by ___%
Hgb: 1g/dL
Hct: 3%
(1:3)
This is the cold insoluble precipitate that forms when a unit of FFP is thawed between 1C and 6C. It contains most of the coagulation factors that are found in FFP.
Cryoprecipitated Antihemophilic Factor (AHF) / CRYO
Light spin utilizes short time and low RPM which yields __.
PRP (platelet-rich plasma)
Heavy spin utilized longer spin and high RPM which yields __.
Concentrated component
Component prepared for a patient with sickle cell disease
RBCs
Component prepared for a patient with thrombocytopenia
Platelets
Component prepared for a patient with Hemophilia A
Factor VIII Concentrate
Component prepared for a patient with Fibrinogen Deficiency
Cryoprecipitated AHF
Eight units of platelets were pooled in an open system without the use of a sterile connecting device (open system). What is the new time of expiration of the pooled product?
4 hrs.
How many platelets must be obtained in a plateletpheresis in order to meet acceptance criteria?
3.0x10^11
Shipping temperature for RBCs
1-10C
ABO incompatibility is acceptable for what component/s?
Cryoprecipitated AHF
