Complete Blood Count [CBC] Flashcards
Congenital heart disease will increase or decrease RBC count
Increase
The most common diluent in RBC counting
Isotonic saline
The most preferred diluent in RBC counting
Davies
An apparatus used in the gasometric method.
This is used to determine what
Natelson microgasometer
The most reliable and recommended method to determine hemoglobin
Cyanmethemoglobin
Another name for the cyanmethemoglobin method
Hemiglobincyanide
Myeloma protein, abnormal globulins, and radiographic media may affect the accuracy of what hemoglobin determination method
Copper sulfate method (specific gravity)
What type of hemoglobin is not measured in the hemiglobincyanide method ?
Sulfhemoglobin
Blood sample and anticoagulant used in the cyanmethemoglobin method
Whole blood, EDTA
What increases the alkalinity when abnormal globulins are present in px sample
Potassium carbonate
The absorbance of the solution in the hemiglobincyanide method is measured in how many nanometers using the spectrophotometer
540 nm
How do you resolve presence of hemoglobin C and S during CBC
Dilute with equal parts water and multiply the result by 2
Reagent for the cyanmethemoglobin method
Modified drabkins
Reagent for the sahli’s acid hematin method
Drabkin’s solution
True or False
- Hematocrit reflects the concentration of RBC
- Hematocrit reflects the total red cell mass
- T
2. F
The reference indicator for calibration blood counting systems
Hematocrit
Increased or Decreased hematocrit
1. Dehydration
Increase - relative concentration due to less plasma
An over-anticoagulated sample results in an ________ (high or low)hematocrit
Low
To obtain the packed cell volume: we set the centrifuge at _____ rpm for ____ minutes
10,000 to 15,000 rpm
5 min
Improper flushing of an IV catheter will result to a false _____ (increase or decrease) in hematocrit value
False decrease
How many ml is the capacity of a capillary tube
0.5 mL
How much (in cm) should you fill a capillary tube when measuring Hct
5 cm
Reference range for MCH
24-34 pg
Sample used in platelet count
Whole blood EDTA
1% ammonium oxalate reagent used in PLT count is use to ______
Lyse RBC
Reese-Ecker method for PLT counting uses:
- Reagent ______
- Stain _______
- Microscope_____
- Citrate formaldehyde (buffer)
- Brilliant cresyl blue
- Phase contrast
Guy and leaks method in PLT count uses:
- Reagent _____
- Stain _____
- Microscope ______
- Oxalate formaldehyde
- Crystal violet
- Light microscope
In PLT estimating, how many OIO fields do we count ______ and how many RBCs _____
10 fields
200 RBCs
Hemolytic Uremic syndrome may cause a false ______ (increase or decrease) in PLTs
Decrease
What chemical found in the modified drabkins reagent that converts methemoglobin to cyanmethemoglobin
Potassium cyanide
What chemical in modified drabkin’s makes ferrous iron become ferric
Potassium ferricyanide
The name of the chemical method in determining hemoglobin
Kennedy and Wong
The hypochromia grading when the central pallor is 3/4 the cell
3+
2+ hypochromia grading indicates ____central pallor
2/3
True or False:
1. Absorbance is INDIRECTLY proportional to the hemoglobin concentration
False
How many percent of adult hemoglobin may be inactive. Hence may not be accurately measure in the gasometric method
2-12%
Using a patient blank (px sample + hemiglobincyanide reagent) resolves what condition
Lipemia
How many WBC and PLT in a sample needs to be centrifuged and re-read again (reading the supernatant)
> 20 wbc
> 700 plt
What reagent is used to speed up testing in the cyanmethemoglobin method
Dihydrogen potassium
