Common Anaerobic Media

Question 1

May be prepared with Columbia, Schaedler, CDC,
Brucella, or brain-heart infusion base supplemented
with 5% sheep blood, 0.5% yeast extract, hemin,
L-cystine, and vitamin K1.

Answer 1

Anaerobic blood agar (Ana BA)

Question 2

Nonselective medium for isolation of anaerobes
and facultative anaerobes (Primary Purpose)

Answer 2

Anaerobic blood agar (Ana BA)

Question 3

Trypticase soy agar base with ferric ammonium citrate
and hemin; bile salts and gentamicin act as inhibitors.

Answer 3

Bacteroides bile esculin agar (BBE)

Question 4

Selective and differential for Bacteroides fragilis
group; good for presumptive identification (Primary Purpose)

Answer 4

Bacteroides bile esculin agar (BBE)

Question 5

Brucella agar base with kanamycin (75 μg/ mL),
vancomycin (7.5 μg/mL), vitamin K1 (10 μg/mL), and
5% laked blood.

Answer 5

Laked kanamycinvancomycin (LKV)

Question 6

Selective for isolation of Prevotella and
Bacteroides spp. (Primary Purpose)

Answer 6

Laked kanamycinvancomycin (LKV)

Question 7

Nutrient agar base, 5% blood, phenylethyl alcohol.

Answer 7

Anaerobic phenylethyl alcohol agar (PEA)

Question 8

Selective for inhibition of enteric gram-negative
rods and swarming by some clostridia (Primary Purpose)

Answer 8

Anaerobic phenylethyl alcohol agar (PEA)

Question 9

Egg yolk base.

Answer 9

Egg yolk agar (EYA)

Question 10

Nonselective for determination of lecithinase
and lipase production by clostridia and
fusobacteria (Primary Purpose)

Answer 10

Egg yolk agar (EYA)

Question 11

Egg yolk base with fructose, cycloserine (500 mg/L),
and cefoxitin (16 mg/L); neutral red indicator.

Answer 11

Cycloserine cefoxitin fructose agar (CCFA)

Question 12

Selective for Clostridium difficile (Primary Purpose)

Answer 12

Cycloserine cefoxitin fructose agar (CCFA)

Question 13

Solid meat particles initiate growth of bacteria; reducing
substances lower oxidation-reduction potential (Eh).

Answer 13

Cooked meat (also called chopped meat) broth

Question 14

Nonselective for cultivation of anaerobic organisms;
with addition of glucose, can be
used for gas-liquid chromatography (Primary Purpose)

Answer 14

Cooked meat (also called chopped meat) broth

Question 15

Peptone base, yeast extract, glucose, cysteine (reducing
agent), resazurin (oxygen tension indicator), salts.

Answer 15

Peptone–yeast extract–glucose broth (PYG)

Question 16

Nonselective for cultivation of anaerobic
bacteria for gas-liquid chromatography (Primary Purpose)

Answer 16

Peptone–yeast extract–glucose broth (PYG)

Question 17

Pancreatic digest of casein, soy broth, and glucose to
enrich growth of most bacteria. Thioglycollate and
agar reduce Eh. May be supplemented with hemin
and vitamin K1.

Answer 17

Thioglycollate broth

Question 18

Nonselective for cultivation of anaerobes,
facultative anaerobes, and aerobes (Primary Purpose)

Answer 18

Thioglycollate broth