Column Quality Flashcards
Resolution equation
R = 2 x delta rt / delta base width
Unit less
Acceptable resolution values
An r value less than five and greater than 1.5 is considered good resolution
A bigger resolution numbers indicates what
Better separation
Higher r values
Efficiency equation
TWO EQUATIONS
One includes retention time and peak width at half peak height
efficiency counts the number of the plates
Plates per metre calculations
Efficiency ie number of plates / column length in metres
How would you show the parameters on a chromatogram that you need to calculate resolution
Retention time and width will allow you to calculate it
How would you show the parameters on a chromatogram that you need to calculate the number of theoretical plates
Retention time
Width at half peak height
CONE BACK
Efficiency and resolution formulas
Come back
What is void time