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Test Histo 3 > Colo > Flashcards

Colo Flashcards

1
Q

Hematoxyline colore quoi

A

Noyau

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2
Q

Nucleol:% proteine

A

80-90%

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3
Q

Nucleol:basophile aime colo…

A

Colorant basique

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4
Q

Nucleol:acidophile colo…

A

Colore avec eosine

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5
Q

Plus d’un nucleol par noyau dans…

A

Cellule active

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6
Q

Materiel colorer du noyau

A

Chromatine

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7
Q

2 type de chromatine

A

Hererochromatine
Euchromatine

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8
Q

Type de chromatine colorable

A

Heterochromatine

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9
Q

Type chromatine non colorable

A

Euchromatine

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10
Q

La quantiter de chaque type de chromatine depend de…

A

Du niveau d’activiter de la cellule

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11
Q

Mecanisme physique de colo

A

Absorption
Adsorption
Prositer

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12
Q

Mecanisme chimique colo

A

Liaison chimique
Liaison hydrogene
Liaison covalente
Van des waals

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13
Q

Mecanisme de colo pour la plus part des colo

A

Plusieue mecanisme en cause

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14
Q

Si une structure est (+) elle est…

A

Basique

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15
Q

Les structure basique aime les colo…

A

Acide

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16
Q

Les colo acide son …

A

(-)

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17
Q

Structure (+) qui aime colo acide(-) est…

A

Acidophile

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18
Q

Structure acidophile:

A

Cytoplasme
Muscle
Collagene
GR

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19
Q

Si une structure est (-) elle est….

A

Acide(-)

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20
Q

Structure (-) aime colo

A

Basique(+)

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21
Q

Structure (-) qui aime colo(+) est

A

Basophile

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22
Q

Structure basophile:

A

Noyau(ARN/ADN)

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23
Q

Les proteine son composer de..

A

Acides amines

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24
Q

Acides amines contiennent des radicaux…

A

Basique charge(+)(amine:NH3+)
Acides charge(-)(carboxyle:COO-)

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25
Q

Proteine avec 2 groupement radicaux son des

A

Amphoteres

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26
Q

La plupart des colo sont composer de

A

Sels complexe demontrant des tendance acide et/ou basique

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27
Q

Radicaux colo

A

NH3+, COO-, SO3-

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28
Q

Colorant qui possede un groupe + et - est apeler

A

Amphotere

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29
Q

Proceder colo directe

A

Iiaison du colorant facon naturel
Pas besoin de substance exterieure

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30
Q

Colo direct exp

A

Eosine: (colo acide-) —> se lie naturellement au cytoplasme (basique +)

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31
Q

Proceder colo indirecte

A

Colo pas d’affinite naturel pour la structure
Besoin d’un ´´trait d’union’´ pour liaison(mordant)

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32
Q

Principaux mordant

A

Cr, Al, Fe, SO4

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33
Q

Compexe mordant-colorant

A

Anionique et non cationique

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34
Q

Mordant qui fait parti du fixateur

A

Acide picrique
Bichromate

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35
Q

Mordan appliquer avant le colorant

A

Alun de Fe avec hematoxyline

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36
Q

Mordant qui est melanger au colorant

A

Harris

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37
Q

Agent de retention principe

A

Retient un colorant qui est deja fixe sur un element
Empeche decoloration

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38
Q

Exp d’agent de retention

A

Iode dans methode de verhoeff

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39
Q

Agent de rehaussement et accelerateur principe

A

Augmente le pouvoir/selectiviter de certain colorant

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40
Q

Coloration progressive principe

A

Colo interompu lorsque structure on atteindu la teinte voulu

41
Q

Colo regresive principe

A

Sur coloration et decolorer avec solvant

42
Q

Defferation par exces de mordant

A

Mordant entre en competision pour la molecule colorante , le colorant se detache du tissu

43
Q

Differentiation par exces de mordant exp

A

Veroeff pour fibre elastique

44
Q

Deffrentiation par acide principe

A

Bris de liaison entre mordant-tissu

45
Q

Differentiation par acide exemple

A

H&E differentiation hemateine

46
Q

Composition noyau

A

Groupement phosphate ADN(-)
Histones(+)

47
Q

Types anions tissulaire

A

Groupement phosphate(acide nucleiques)PO4,PO3-
Groupement esters(sulfatés)SO4, SO3-
Groupement carboxyle(glucide)COOH,COO-

48
Q

Colo de tou groupement cationique pH

49
Q

Groupement sulfater pH color:

A

1,0
2,5-3,0
4-5
>5

50
Q

Groupement phosphate pH color

A

2-5-3,0
4-5
>5

51
Q

Groupement carboxyle pH color

52
Q

Si le pH continu a augmenter la colo devien..

A

Moin specifique

53
Q

PH plupart colo nucleair cationique

54
Q

Colo adequat seul ou contrecolo

A

Bleu toluidine O
Rouge neutre
Vert methyle
Safranime O

55
Q

Acide amine non dissocie

56
Q

Acide aminee basique (pH1,0)

A

NH3+, COOH

57
Q

Acide amine neutre(pH6,0)

58
Q

Acide aminer acide(pH14)

59
Q

Acide amine courant de liaison du colorant

A

Arginine, histidine, lysine

60
Q

Colorant ou contrecolorant le plus utiliser

61
Q

Eosine pH

A

7 mais colo doit etre faite plus bas que 6

62
Q

Si le pH est endessou de 4 l’eosine seras transformee en

A

Acide libre

63
Q

Acide libre resulta

A

´´muddy’´

64
Q

Eosine meilleur pH

65
Q

Si eosine utiliser correctement on obtein

A

3 ton de rose

66
Q

Chromophore responsable de..

A

Couleur de la molecule

67
Q

Auxochrome responsable de..

A

Attache su substrat et modifi absorbtion de longueur d’onde

68
Q

C=C done:

69
Q

C=O done:

70
Q

C=S done:

71
Q

C=N done:

72
Q

N=N done:

73
Q

N=O done:

74
Q

NO2 done:

75
Q

Quel es la forme la plus simple d’un composer aromatique

76
Q

Formul chimique benzene

77
Q

Colorant ayant une charge nette positif=

A

Colorant basique ou cationique

78
Q

Colorant ayan une charge negative=

A

Colorant acide ou anionique

79
Q

Auxochrome basique

A

Primaire
Secondaire
Tertiaire
Quaternaire

80
Q

Auxochrome acide

A

Sulphonic
Carboxylique
Phenolique/Hydroxyle

81
Q

Acide[ ] sont fort mes a pH bas

A

Sulfonique

82
Q

Acide [ ] sont faible et partiellement ionise en solution aqueuse

A

Carboxylique

83
Q

Plupart [ ] sont plus faible que carboxylique

84
Q

Formation molecule colo

A

Benzene(incolore)
3NO2(chromophore)
Trinitrobenzene(pas encore colo)
OH(auxochrome)
Acide picrique(colo)

85
Q

Classification colo critere

A

Origine colo(naturel ou synthetique)
Methode d’application
Type chromophore
Color index

86
Q

Hematoxyline vien de ou

87
Q

Pk hematoxyline nest po un colo

A

Pas de chromophore
Doit etre oxyder
A besoin d’un mordant

Devien hemateine

88
Q

Carmin provient …

A

Cochenille femelle

89
Q

Carmin caracteristique

A

Couleur rouge
C22H20O13
Doit avoir mordant
Amphotere

90
Q

Orceine provenence

91
Q

Orceine caracteristique

A

Incolore(besoin oxydation)
Bleu violacer

Met en evidence fibre elastique et chromosome

92
Q

Safran provenence

A

Plante(crocus sativus)

93
Q

Safran caracteristique

A

Jaune
Acide
Fibre collagene

94
Q

Methode aplication et type auxochrome colo basique

A

Colo cationique-charge positive-auxochrome amine
Exp:fuschine basique

95
Q

Methode aplication et type auxochrome

A

Colo anionique-charge negative-auxochrome acide
Eosine

96
Q

Colo neutre=

A

Colo acide et basique

97
Q

Colo neutre son habituellement utiliser pour

A

Utiliser en solution acide pour rehausser l’ionisation

98
Q

Ordre force aciditer

A

Sulfate-phosphate-carboxyle-hydroxyle

Test Histo 3 (3 decks)

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