Collection of Bacterial Samples. Flashcards
Should we try to collect bacterial samples before or after antibiotic treatment has been administered?
Before antibiotic treatment has been administered.
What must we ensure does not happen to our bacterial sample if it is to remain viable?
We must make sure that it does not get contaminated.
What are 2 ways that help us to prevent contaminating our bacterial sample?
By cleaning the area from where we are taking the sample.
By making sure the equipment we use is sterile.
What are the best samples to take from the animal?
Fluids.
What should happen to samples once they have been collected from the animal?
They should be kept moist and at a constant temperature of 4 degrees C.
Why should bacterial samples be kept at a temperature of 4 degrees C?
Because the bacteria will remain alive, but will not multiply.
Why are dry swabs not the best media to use for taking bacterial samples?
As the bacteria will not survive for very long on the dry media.
What dictates the methods that we use to remove a bacterial sample?
On what we suspect the disease is.
What dictates how much tissue we take for a bacterial sample?
What we suspect the disease is.
What are the 5 main sample types that we will obtain from animals?
Fecal.
Blood.
Skin.
Sputum.
Urine.
What do we generally use to identify different bacterial infections?
Different diagnostic kits.
What are the 2 methods that we can use to identify unknown bacteria in a sample?
Direct methods.
Indirect methods.
What are the 3 steps in the direct method of identifying bacteria?
Identify bacteria via microscopy.
Culture bacteria.
Carry out antimicrobial sensitivity test on cultures.
What are the indirect methods of identifying bacteria?
They involve detecting antibodies and antigens within the blood.
When will we obtain tissue samples from an animal for analysis?
Only at post mortem.
