COAGULASE NEGATIVE STAPHLOCOCCI ( EPIDERMIS & SAPROPHYTICUS)

Question 1

CLASSIFICATION for both

Answer 1

gram + cocci
clusters
non sporing
non motile
non capsulating

Question 2

BIOCHEM/PHYSIO for both

Answer 2

catalase+
coagulase -
oxidase -
facultative anerobe

Question 3

PATHOGENESIS/PATHOGENCITIY for EPIDERMIS

Answer 3

S.epidermis is part of the normal flora of the skin and anterior nare
important cause of infection is by prosthetic implants
- surgical implants such as heart valves and catheters are easily infected
- cell envelope polysacharide enable biofilm formation which facilitates attachment to plastic surfaces
- biofilm forming ability acts as an important virulent factor.

Question 4

PATHOGENESIS/PATHOGENCITIY for SAPROPHYTICUS

Answer 4

part of normal vaginal flora
cysitis in women - a urinary coagulase negative staphlococci is presumed to be saprophyticus and can be confirmed with novobiocin resistance.

Question 5

TREATMENT/prevention for EPIDERMIS

Answer 5

for penicillin G isolates: oxacilin & nafcillin
for methicillin resistant: vancomycin
no preventative drug or vaccine available

Question 6

TREATMENT/prevention for saprophyticus

Answer 6

ciprofloxacin

no preventative drug or vaccine available

Question 7

IMMUNITY

Answer 7

NA

Question 8

LABORATORY ID

Answer 8

gram + cocci
cultivation: BA 10% nacl colonies 1-2mm
coagulase negative
and then sapro is novobiocin resistant.