Clotting Factors and Anticoagulants

Question 1

List and describe the domains of FVIIa

Answer 1

1. Gla domain: phospholipid binding
   Defines vitamin K dependent proteins (FII, FVII, FIX, FX and PC, PS) which contain 9-11 γ–carboxyglutamic acid residues
2. 2x EGF-like domain: important for protein-protein binding
3. Serine protease: contains catalytic triad Ser/ His/ Asp which which catalyses proteolysis of target substrate (FIX to FIXa and FX to FXa)

Question 2

Describe the structure of TFPI

Answer 2

• 3 Kunitz domains

Question 3

Describe the structure of TFPI

Answer 3

• 3 Kunitz domains
• Kunitz 2 must inhibit FXa first
• ## Kunitz 1 inhibits FVIIa