clinical microbiology Flashcards
list three things that pathogenicity of an organism is dependent upon
location within the host’s body, host’s immune status, pathogen’s genetic makeup
list one pathogenic strain of E coli
E coli 0157:H7
why is E coli 0157:H7 pathogenic
contains the gene for the shiga toxin and has the p0157 plasmid that contains a gene involved in adhering the bacterium to intestinal cells
list some ways that a virulence factor increases the virulence of an organism
helps it evade host’s immune defenses, allows it to be more transmissible + to attach and colonize a host + gain nutrients within a host + cause damage within a host
list 3 features of PAIs
found in both gram pos and neg, found in the conserved regions of the chromosome, have a different GC content of the host chromosome
describe how to name restriction enzymes using EcoR1 as an example
E = genus, co = species, R = strain, 1 = how many have been isolated
what type of media is chocolate agar
nonselective + enriched
what type of media is blood agar
enriched + differential (differentiates between types of hemolysis)
what type of media is Hektoen agar
selects for gram neg enteric rods
differentiates between Salmonella sp. and Shigella sp.
purpose of API 20E and enterotube system?
identify members of the family Enterobacteriaceae
what does MALDI-TOF MS stand for
matrix assisted laser desorption ionization time of flight mass spectrometry
what does CNA agar contain
blood agar with colistin and nalidixic acid
what type of agar is CNA agar
enriched + differential (hemolysis), and it selects for gram pos
what type of agar is MacConkey agar
selects for gram negative, differentiates between lactose fermenters
how does alpha hemolysis appear
green product
