Clinical Chemistry

Question 1

Metabolite of Catecholamine

Answer 1

VMA (Vanillyl Mandellic Acid)

Question 2

Measurement of 17OH corticosteroid

A. ziemmermann
B. Porter Silber Reaction
C. Pisano Reaction
D. Kober Reaction
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Answer 2

B. Porter Silber Reaction

Ziemmermann- 17KS
Pisano Reaction- VMA measurement
Kober- estrogen, estriol, estradione

Question 3

Major Cation in ECF

Answer 3

Sodium

Question 4

Hypernatremia is observed in:

A. Overhydration
B. Use of diuretic
C. Aldosterone deficit
D. None

Answer 4

D. None

Overhydration- increase water:dilution of Na
Use of diuretic- increase excretion of Na
Aldosterone deficit-
(increase aldosterone=increase reabsorption of Na=increase Na ; decrease aldosterone=decrease reabsorption=decrease Na

Question 5

Test for HEAVY METAL POISONING

Answer 5

Reinsch Test

Question 6

Buffer Inside Red Blood Cell

Answer 6

Hemoglobin

Question 7

Increase pCO2; Normal HCO3; decrease pH

Answer 7

Respiratory Acidosis

Question 8

50% of Calcium exist in:

A. Free or Ionized
B. Bound to Plasma protein
C. Complex form
D. None

Answer 8

A. Free or ionized

99%: bone and teeth
1%: blood
      -50% free/ionized
      -40% albumin bound
      - 10% complex as hydroxy apatite

Question 9

Lowers serum Calcium level

A. Active Vit d3
B. PTH
C. Calcitonin
D. GH

Answer 9

C. Calcitonin

Question 10

Most common cause of increase plasma enzyme

Answer 10

Released from the death of enzyme containing cell

Question 11

Best temperature to measure enzyme activity?

Answer 11

37 degrees C

Every 10degrees rise in temp=reaction rate doubles
56degrees- denatures enzyme
60degrees- preciptate enzyme

Question 12

ACP band inhibited by tartrate but not by copper ion?

A band 1
B band 2 and 4
C band 3
D band 5

Answer 12

A. Band 1

2 main isoenzyme Copper Tartrate

30% RBC (band 1). Adsorbed. ——

70% prostate (band 2). —- Adsorbed

Question 13

Optimal pH of ACP

Answer 13

9.8

Question 14

Immediate precursor of unconjugated bilirubin

Answer 14

Biliverdin

Question 15

Only test to evaluate conjugation function

Answer 15

Hippuric or quick’s test

Question 16

Monitor hepatic coma

Answer 16

Ammonia

Question 17

Floating beta lipoprotein

Answer 17

Beta-VLDL

Question 18

Major component of of HDL

Answer 18

Apo A

Apo A1- HDL
ApoB100-LDL
ApoC1-VLDL
ApoE- alzheimer’s

Question 19

Best anticoagulant for LIPID

Answer 19

EDTA

Question 20

Urea is derived from______ metabolism

Answer 20

Protein metabolism

Question 21

Synthesis of urea in the liver is catalyzed by what enzyme system?

Answer 21

Ornithine carbamyl tranferase

Question 22

Direct method of urea

A. Is measured by nessler’s reaction
B. Yields value multiplied by 2.14
C. Measures BUN
D. Utilizes diacetyl monoxime reagent

Answer 22

D.

Question 23

Normal value of Urea

Answer 23

8-20mg/dL

Question 24

Metabolite of Cocaine

Answer 24

Benzoylecgonine

Question 25

Metabolite of dopamine

Answer 25

homovanillic acid

Question 26

Differentiates pheochromocytoma from neurgoenic hypertension

Answer 26

Clonidine test

Glucagon test- highly suggestive of pheochromocytoma