CLINICAL CHEMISTRY 2: ENZYMOLOGY

Question 1

Factors affecting Enzymatic Reactions

Answer 1

Substrate Concentration
Enzyme Concentration
Cofactors
Inhibitors
Hydrogen Ion concenration or pH
Storage
Hemolysis
Lactenscence or Milky specimen

Question 2

Optimum temp for enzymatic reactions

Answer 2

37C

Question 3

3 types of Creatine Kinase

Answer 3

CK-MM (Muscle type)
CK-MB (Hybrid type)
CK-BB (Brain type)

Question 4

What are the types of Lactate Dehydrogenase

Answer 4

LD-1 (HHHH)- Heart and RBCs
LD-2 (HHHM)- Heart and RBCs
LD-3 (HHMM)- Lungs, Lymphocytes, Spleen, Pancreas
LD-4 (HMMM)- Liver, Skeletal muscle
LD-5 (MMMM)- Liver, Skeletal muscle