Clinical Chemistry Flashcards

1
Q

comes from the greek word kline, meaning
“bed”

A

clinical

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2
Q

the science that deals with the elements,
their compounds and the chemical structure and
interaction of matter

A

chemistry

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3
Q

Study of biochemical processes associated with
health and disease

A

Clinical chemistry

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4
Q

few and scanty, indeed are the rays of light which chemistry has flung
on the vital mysteries

A

Robert James Graves (1796-1853)

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5
Q

complained that clinicians do not use their chemistry laboratory services except when needed for luxurious
embelishment for a clinical lecture

A

Max Josef von Pettenkofer (1818-1901)

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6
Q

First device to measure acidity of citrus fruits

A

pH meter

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7
Q

a device that would
automatically pipette a sample, add reagents to it,
mix and incubate the resulting solution and measure
the color change with a colorimeter

A

colorimeter

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8
Q

composed of solvent and solute. In a
solution, there is an even distribution of molecules or
ions throughout the solvent

A

solution

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9
Q

refers to the substance being dissolved which
maybe a solid, liquid or gas

A

Solute

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10
Q

refers to the substance in which the solute
is dissolved which most cases are liquids

A

Solvent

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11
Q

refers to the weight or volume of the solute
present in a specified amount of the solvent or a
solution

A

Concentration

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12
Q

amount of solute in a solution can be measured as a
percentage of the total volume of the solution

A

Percent solution

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13
Q

solution containing one gram molecular weight (one mole
of the solute in one liter solution) of the substance per liter
of the solution
The number of moles expressed per 1 liter of solution.

A

Molar Solutions (MOLARITY)

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14
Q
  • one gram equivalent weight of solute in one liter of
    solution
  • Gram equivalent weight is that weight in grams of an
    element or compound which will combine with or
    replace one gram of hydrogen
  • It has often been used in acid-base calculation
A

Normal Solutions (NORMALITY)

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15
Q
  • Amount of solute per 1kg of solvent
  • It is expressed as moles per kilogram (mol/kg) or
    weight/weight
A

molality

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16
Q

most common way of expressing electrolytes
the equivalent weight expressed in milligrams.

A

MILLIEQUIVALENTS

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17
Q
  • In the molar, normal, or percentage solutions,
    the amount of solute contained in a given
    volume of solution is equal to the product of
    volume times the concentration
A

Dilution

18
Q

These chemicals are sufficiently pure to be used in
many analyses in the clinical laboratory

A

Chemically Pure (CP) Grad

19
Q

These reagents meet the specifications stated in USP and NF.

A

United States Pharmacopeia (USP) and
National Formulary (NF) Grade

20
Q

These chemicals may be used as starting
materials for synthesis of other chemicals
of greater purity but generally should not
be used in the clinical laboratory

A

Purified, Practical of Pure
Grade

21
Q

These chemicals are used only for
industrial purposes and are generally
not used in the preparation of
reagents for the clinical laboratory

A

Technical or Commercial
Grade

22
Q

¢Used for test methods requiring minimum
interference.
¢For procedures that require maximum water impurity
for accuracy and precision.
¢Uses: flame photometry, AAS, blood gases and pH
enzyme studies. Electrolyte testing, HPLC, trace metal
and iron studies

A

Type 1 reagent water

23
Q
  • For hematology, microbiology ,immunology
    and chemistry.
  • Acceptable for preparation of reagents and
    quality control materials.
A

Type II reagent Water

24
Q
  • Urinalysis, parasitology and histology
  • For washing glasswares.
A

Type III Reagent water

25
Q

is the condesate collected from
steam and created when water is boiled and
vaporized. It has been purified to remove almost all
organic materials

A

DISTILLED WATER

26
Q

It is prepared by using deionizer
(anion or cation) and it is free from minerals salts;
removed by ion exchange processes.

A

DEIONIZED WATER

27
Q

essentially a sodiumaluminum borosilicate with an excess of silica
characterized by high degrees of thermal resistance.

A

Borosilicate glassware

28
Q

This is comparable to fused quarts in its thermal
endurance chemical stability and electrical
characteristics. It is radiation resistant and has good
optical qualities and temperature capabilities. It is used
for precision analytical work and can also be used for
optical reflectors and mirrors.

A

High silica glass (over 96%)

29
Q
  • This is used to handle strongly alkaline
    solutions. However, it has only about half the
    thermal block or rather shock resistance of
    Pyrex glassware and therefore must be heated
    and cooled more carefully.
  • Often referred to as “soft glass as its thermal
    resistance is much less than of borosilicate
    glass.
A

Glass with high resistance to alkali

30
Q
  • It is a glass of high thermal with a red color added as an integral
    part of the glass.
  • Used for the manufacture of weighing bottles because it
    develops less static surface changes
A

Standard flint glass

31
Q

This is soda-lime glass composed of a mixture of
the oxides of silicon, calcium and sodium. It is
lowest in cost and is readily fabricated in a wide
variety of shapes. It has poor resistance to high
temperature and sudden changes of temperature
and its resistance to attack by chemicals is only
fair

A

Low actinic glass

32
Q

commonly called graduates
and are used where less accurate measurements are
required

A

GRADUATED CYLINDER

33
Q

are long cylindrical graduated pipettes
with stopcock(glass for acid and rubber for alkali).
This is generally used for titration purposes only.

A

BURETS

34
Q

are long cylindrical graduated pipettes
with stopcock(glass for acid and rubber for alkali).
This is generally used for titration purposes only.

A

BURETS

35
Q

are frequently used for the
preparation of standard solutions (solution of known
concentration) and for measuring liquid volume
accurately

A

VOLUMETRIC FLASKS

36
Q

there are many kinds of pipets available for
the use in clinical chemistry laboratory each intended
to serve specific function

A

PIPETS

37
Q

this type of calibration is made by
weighing the necessary volume of water which when
allowed to flow by gravity will deliver the exact volume.

A

To deliver (TD)

38
Q

Are calibrated by introducing exact volume or
weight of mercury(Hg), a non-wetting liquid, equal
to volume desired. The pipet contains the
necessary volume, howerer, it does not deliver the
exact volume due to the tendency of the fluids
cling to glass surfaces.

A

To contain (TC) pipets

39
Q
  • -same as TD pipette but drops remaining at tip after
    delivery to blown out to receiving vessel.
    • an etched ring/ frosted edge is seen near the
      mouthpiece.
  • self pipette
  • -the user allows the contents of the
  • Pipette to drain by gravity
A

BLOW-OUT PIPETTE

40
Q

first step in sample collection

A

“PROPER PATIENT IDENTIFICATION

41
Q

nothing by mouth is what type of collection?

A

Fasting

42
Q
  1. general fasting time
  2. glucose
  3. triglycerides
A
  1. 8 - 14hrs
  2. at least 8 hours
  3. 10-12 hrs