Clicker Questions

Question 1

Which of these polymerases does NOT transcribe rRNAs and tRNAs?

A. RNA pol I
B. RNA pol II
C. RNA pol III
D. Bacterial RNA
polymerase

Answer 1

B. RNA pol II

Question 2

What would be the consequence of a null
mutation in the guanylyltransferase that caps
the mRNA?

Answer 2

mRNAs would not
be translated

Question 3

Which one of these genes has the highest transcription rates?

Answer 3

RecA

Question 4

If the coding strand is the following sequence,
what is the mRNA sequence?

5’ ATGCAGTCCATA

Answer 4

5’ AUGCAGUCCAUA

Question 5

If the template strand is the following sequence,
what is the mRNA sequence?

5’ ATGCAGTCCATA

Answer 5

3’ UACGUCAGGUAU

Question 6

What would be the consequence
of a null mutation in TFIIH?

Answer 6

open complex does
not form

Question 7

Which of the following is not a difference
between prokaryotic and eukaryotic
transcription?

Answer 7

open complex formation
precedes elongation

Question 8

Which of the following mutations would most
likely happen if you got an x-ray without a lead
shield?

Answer 8

chromosomal break

Question 9

What mutagen most commonly
causes thymine dimers?

Answer 9

sunlight

Question 10

What would be the consequence
of a mutation in MutH?

Answer 10

Increased mutations
after DNA
replication

Question 11

What kind of mutation commonly
occurs after NHEJ

Answer 11

small deletions

Question 12

What type of mutation is shown in the following:
5’ ATGCAGTTTAAG
5’ ATGCAGATTTAAG

Answer 12

frameshift

Question 13

What type of mutation is shown in the following:

5’ ATGCAGTTTAAG
5’ ATGCAGTTCAAG

Answer 13

silent

Question 14

Which of these mutations would
likely have the most severe impact on the phenotype?

Answer 14

mutation in splice
junction

Question 15

If this sequence has deamination of 5mC, what would be the resulting mutation?

5’ – ATGCCGTAT -3’

Answer 15

missense

Question 16

Which of these elements require
transposase to move?

Answer 16

Insertion elements
Simple transposons

Question 17

What would be the consequence of a nonfunctional DNA ligase during DNA replication?

Answer 17

Single-stranded breaks in
DNA would be present

Question 18

Which of the following is a difference between prokaryotic and eukaryotic DNA replication?

Answer 18

Ends replication problem
Flap endonuclease

Question 19

Experimental results support which model(s) for initiating recombination during meiosis and DNA repair?

Answer 19

DNA double strand breaks (DSB)

Question 20

Which pair of proteins is required for nucleotide excision repair (NER)?

Answer 20

UvrC and DNA ligase

Question 21

Double-strand breaks are typically repaired by which of the following?

Answer 21

nonhomologous end joining and homologous recombination repair

Question 22

Bases which exist in keto and enol or amino and imino forms are best described as ______.

Answer 22

tautomers

Question 23

Alkylating agents include nitrogenBlank 1 gas and ethyl Blank 2 (EMS).

Answer 23

mustard
methanesulfonate

Question 24

Proflavin is best described as a(n) ______ agent.

Answer 24

intercalating

Question 25

base analogues include

Answer 25

5-bromouracil
2-aminopurine

Question 26

The Ames test uses a strain of the bacteriumSalmonella typhimuriumthat is unable to produce the amino acid

Answer 26

histidine