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CK Flashcards

1
Q

Total creatine kinase-Kinetic method

A

Oliver-Rosalki, modified by Szasz

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2
Q

Addittion of Sulfhydryl and AMP

A

Nielson

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3
Q

Optimized the condition for measuring CK activity

A

Szasz and Scandinavian committee

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4
Q

Recommended method of IFCC

A

Reverse reaction and activation of NAC

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5
Q

pH of forward reaction

A

pH 6.8

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6
Q

pH of reverse reaction

A

pH 9.0

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7
Q

Activator of CK

A

Magnesium

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8
Q

Enzymes of forward reaction

A

CK, Pyruvate Kinase, Lactate dehydrogenase

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9
Q

Enzymes of reverse reaction

A

CK, Hexokinase, Glucose-6-Phosphate Dehydrogenase

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10
Q

Final product of forward reaction

A

NADH

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11
Q

Final product of reverse reaction

A

NAD

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12
Q

Direct or forward reaction method

A

Tanzer-Gilbarg method

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13
Q

Methods of separation of isoenzyme

A

Electrophoresis
Immunoassay
Column chromatography

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14
Q

Excess magnesium, chloride, calcium, sulfates

A

Inhibit CK activity

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15
Q

Heavy metals that inhibits CK activity

A

zinc, copper, manganese

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16
Q

Excess iodoacetate, and sulfhydryl agents

A

Inhihits CK activity

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17
Q

Excess ADP, citrate, fluoride, L-thyroxine, and uric acid

A

Inhibits CK activity

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18
Q

4hrs
8-12hrs
2-3 days

A

RT
4 deg celcius
Frozen

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19
Q

High ALP

A

Decrease CK value

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20
Q

Inactivated by light

A

CK activity

21
Q

Lipemic sample

A

Causes high absorbance readings

22
Q

Intramuscular injection of sedative and analgesics

A

Induce increase CK activity

23
Q

Men have higher CK than women because of difference in?

A

Muscle mass

24
Q

New born high value of CK due to

A

Skeletal muscle trauma during birth

25
Q

Recent exercise

A

Increase serum CK

26
Q

Sustained exercise

A

Increase CK-MB

27
Q

Elders have decrease CK due to

A

Decrease muscle mass

28
Q

Linearity of Total creatine kinase-Kinetic method

A

1200 IU/L

29
Q

Magnesium

A

Cofactor for maximum catalytic activity

30
Q

Adenosine-5’-Monophosphate

A

Inhibits adenylate kinase activity

31
Q

N-acetylcysteine

A

Prevents oxidation of sulfhydryl group

32
Q

Oxidation of sulfhydryl leads to

A

No enzyme activity of CK

33
Q

EDTA

A

Chelates calcium which is an inhibitor of CK

34
Q

55 deg celcius

A

No detectable CK activity

35
Q

Preffered method of reaction

A

Reverse reaction

36
Q

This reaction is 2-6× faster

A

Reverse reaction

37
Q

CK- MB determination Immuno-inhibition

A

Wurzburg

38
Q

Major source of CK

A

Muscle- CK-MM
Heart- CK- MB
Brain- CK-BB

39
Q

Subunits of CK

A

M and B

40
Q

Isoenzyme are separated via

A

Electrophoresis
Column anion exchange
Batch anion exchange chromatography

41
Q

Antibody to CK- M monomer

A

Wurzburg

42
Q

Quantitatively measure CK- B

A

Rosalki

43
Q

Uses precipitating antisera against ( homologous rabbit antisera ) pure CK-MM and CK-BB

A

Immunoprecipitation

44
Q

Used for fast and quantitative measurement of CK-MB

A

Immuno- inhibition

45
Q

Used in doubtful cases

A

Immunoprecipitation

46
Q

Isoenzyme that is usually absent is a healthy sera

A

CK-BB

47
Q

Linearity of CK-MB determination

A

1500 IU/ L

48
Q

Measurement of isoenzyme

A

Radio immunoassay
Ion exchange chromatography
Immuno inhibition
Electrophoresis

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