Chromosome Walking Flashcards

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1
Q

positional cloning

A

wen a gene is cloned based on its mapped position along a chromosomes

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2
Q

what is one method of positional cloning?

A

chromosome walking

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3
Q

what is chromosome walking?

A

a mapped gene or molecular marker provides a starting point from which to molecularly walk toward a gene of interest via overlapping clones

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4
Q

how is chromosome walking initiated?

A

it requires using a gene close to the gene of interest. A small piece of DNA from the neighboring gene is inserted into another vector called sub cloning

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5
Q

what does sub cloning do?

A

a sub cloned piece of DNA is at the end of the insert, so when it is used as a probe, it identifies the second clone closer to the desired gene.

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6
Q

what is library screening?

A

using a subcloned gene as a probe, where the clone is exposed to embers of a cosmid library

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7
Q

how do you know you’re not walking in the wrong direction?

A

when starting at a neighboring gene, markers on the other side of this gene are also identified

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8
Q

how do researchers know when they have reached the gene of interest?

A

in the case of a disease causing gene, researchers would use chromosome walking in an affected and unaffected individual

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