Chromosome Practical Flashcards
How does colcemid work?
By arresting the growth of developed microtubules.
By stopping the formation of microtubules.
What is the FINAL concentration of colcemid in the culture medium?
0.099 ug / ml
KCL- the cells swell when resuspended in 0.075M potassium chloride as an Osmotic Gradient is formed.
True or false?
True!
Quality of chromosome spreads: why did we drop from a height and what would happen if you reduced the height?
To BREAK the cell membranes allowing the chromosomes to spread!
Less chromosome spreads, more intact nuclei.
In the metaphase stained chromosome microscope images, what do the purple stained CIRCLES represent?
Interphase nuclei!
Why are the counts of chromosomes NOT the same?
Some chromosomes are hidden under others.
Some chromosomes LOST through culturing.
You used too much extraction on the slide!
Would you expect the experiment to represent the haploid or diploid number?
Diploid!
