Chromatography

Question 1

Absolute error equation

Answer 1

(mean value of replicates) - (true value)

Question 2

Relative error equation

Answer 2

[(mean value of replicates - true value)/true value] x 100%

Question 3

Recovery equation

Answer 3

[(mean enriched sample value - mean native sample value)/amount added] x 100%

Question 4

Radiolabeled compound recovery equation

Answer 4

[(mean measured label value)/(amount of label added)] x 100%

Question 5

Method LOD equation

Answer 5

mean blank sample + 3 x SD of blanks

Question 6

Method LOQ equation

Answer 6

mean blank sample + 6 x SD of blanks

Question 7

Octanol-Water Partitioning Constant (Kow)

Answer 7

Concentration in octanol/Concentration in water

Question 8

Distribution constant (K)

Answer 8

Concentration in stationary phase/Concentration in mobile phase

Question 9

Velocity of analyte through the column (v)

Answer 9

Length of column/retention time

Question 10

Velocity of M.P. through the column (u)

Answer 10

Length of column/M.P. retention time

Question 11

Retention factor (k’)

Answer 11

(Analyte R.T. - M.P. R.T.)/(M.P. R.T.)

Question 12

Selectivity factor (α)

Answer 12

(k’_B)/(k’_A)

Question 13

Number of theoretical plates (N) equation 1

Answer 13

Length of the column/Height equivalent of a theoretical plate

Question 14

Number of theoretical plates (N) equation 2

Answer 14

16[(Analyte R.T.)/(peak width at the base)]^2 = 5.54[(Analyte R.T.)/(peak width at half height)]^2

Question 15

Plate height (H)

Answer 15

A + B/u +C∙u = A + B/u +(C_mobile + C_stationary)∙u

Question 16

Resolution (R_s)

Answer 16

2 (Peak 2 R.T. - Peak 1 R.T.)/(peak 2 width at the base + peak 1 width at the base)

Question 17

Lambert-Beer’s law

Answer 17

log(I_0/I) = A = εbc