Chromatography Flashcards
1
Q
Why is chromatography able to separate a mixture of compounds? (3)
A
2
Q
What are the 3 types of chromatography?
A
- Thin-Layer Chromatography (TLC)
- Column Chromatography (CC)
- Gas Chromatography (GC)
3
Q
What does separation depend on?
A
4
Q
What is the component of the mixture if its further up the plate? (3)
A
Travels quickly so has low affinity to stationary phase
High solubility in the mobile phase
Less polar
5
Q
Diagram of Thin-layer Chromatography
A
6
Q
Equation to calculate Rf value (Retention Factor)
A
7
Q
Advantages of TCL? (3)
A
8
Q
Limitations of TLC? (5)
A
9
Q
What is Column Chromatography? (3)
A
- Column packed with powder (e.g. silica) to act as stationary phase
- Solvent poured on top of column + soaks down
- Different components travel down at different rates
10
Q
Advantage of Column Chromatography?
A
Larger quantities can be separated
11
Q
What is Gas Chromatography? (2)
A
- Carrier gas (mobile phase) flows through capillary column
- record retention time
12
Q
Limitations of Gas Chromatography? (3)
A
13
Q
How can limitations of Gas Chromatography be overcome?
A
14
Q
Uses of GC-MS? (4)
A
15
Q
QUESTIONS FROM BOOKLET
A
