Chromatography Flashcards
Stationary Phase
A solid, gel or immobilized liquid
Chromatographic bed
A column (glass or metal) or sheet (glass, plastic or paper) used to support the stationary phase
Mobile Phase
A solvent (liquid or gas) which carries samples through the stationary phase
What was the mobile phase of this experiment?
DI water
Delivery system
A force which moves the mobile phase through the stationary phase (gravity or pressure)
Detector
A method of indentifying ot quantifying substances as they elute
What is eluting?
Leaving the stationary phase
What is Chromatography used for?
A scientific techniques used to
1. Seperate
2. Purify
3. Identify
4. and Quantify
… components of a mixture based on different physical characteristics
What physical characteristics can chromatography exam? (4)
- Molecular size
- Shape
- Solubility
- Charge
What is the volume of the resin bed?
50 mL
The glass column can be defined as the…
chromatographic bed
______ bind to the resin.
Cations
What does ion exchange chromatography in this experiment aim at determining?
The concentration of the cations in a sample solution
What is the equation used to describe water passing through the resin?
2R-Na2+ + Ca2+ -> R2-Ca2+ + 2Na+
Cations with a _______ binding affinity will displace cations binded to the resin.
higher
What is the detector of this experiment?
Acid-base titrations
What does Ion exchange chromatography utilize?
The reversible interchange of ions between a solid stationary phase and a liquid mobile phase, with no change to the solid. Mixtures are separated based on the charges of the components
Is the resin the stationary phase?
Yes
What is the standard used in the titration?
NaOH
Eluate
The sample leaving the column
Eluent
The sample loaded onto the column
Elute
The separation process in chromatography
What colour is a solution that contains H+
colourless
How is the resin regenerated?
The resin it is flushed with a huge excess of H+ ions (using large volumes of 6 M HCl), which replaces cations with H+ simply by out-competing them
The many various fields of chromatography can be categorized based on:
1) bed shape
2) Mobile phase
3) Mechanism of separation
