Chromatography Flashcards
What is chromatography useful for
to separate and analyse the substances present in a mixture
What are the two phases of chromatography
- stationary phase
- mobile phase
What is the pattern of bands or spots called
chromatogram
What does the rate of movement of each component depend on
- how strongly the component adsorbs onto the stationary phase
- how readily the component dissolves in the mobile phase
What is adsorption
the adhesion of molecules or substances to the surface of a solid or liquid
What states is the stationary phase in
solid or liquid
What states is the mobile phase in
liquid or gas
What are the three simple forms of chromatography
paper, thin-layer, column chromatography
What is the process of chromatography
Continual process of adsorption onto the solid stationary phase, followed by desorption and dissolving into the mobile phase
What does the ability of the components to stick to the stationary phase depend on?
the polarity of the stationary phase and the component molecules