Chromatography Flashcards
What is the mobile phase
Solvent
Solvent is
Mobile phase
Stationary phase is
What sample is separated on
TLC stands for
Thin layer chromatography
How are substances separated in TLC
•differences in affinities in stationary phase
•differences in solubilities in mobile phase
How are substances separated in column chromatography
•differences in affinities in stationary phase
•differences in solubilities in mobile phase
How are substances separated in gas chromatography
•differences in affinities in stationary phase
Stationary phase in TLC is
Silica plate
Stationary phase in column chromatography is
Silica powder
Stationary phase in gas chromatography is
Silica powder/resin coated within column
Mobile phase in gas chromatography
Inert carrier gas eg N2 or He
How does TLC record
Rf value
How does gas chromatography record
Retention time and relative abundance
Suitable reagent and condition for hydrolysis
Conc HCl
Heat
Why might you need two different solvents
Some compounds may not dissolve in one solvent