Chromatography

Question 1

Chromatography is first developed by the Russian botanist named?

Answer 1

Mikhail Tswett

Question 2

smallest unit of matter; an element consists of an atom, or things that cannot be broken down

Answer 2

Atom and Elements

Question 3

• combined or union of elements or two or more atoms (e.g Hydrogen and Oxygen)

Answer 3

Compound

Question 4

• can either be liquid or gas (moving/mobility)

Answer 4

Mobile phase

Question 5

• firm or not moving; a place wherein compounds are to be separated

Answer 5

Stationary phase

Question 6

• molecule attraction; the compound is adsorbed to a solid support such as silica or alumina.

Answer 6

Adsorption

Question 7

: separates compounds based on their partition between the liquid mobile phase and liquid stationary phase coated on a solid support.

Answer 7

Partition

Question 8

• uses column packing material that has charge-bearing functional groups attached to a polymer matrix.

Answer 8

lon-exchange chromatography

Question 9

• used immobilized biochemical ligands as the stationary phase to separate a few solutes from other untreated solutes.

Answer 9

Affinity Chromatography

Question 10

: separates molecules according to different sizes. As the solute travel through, small molecules enter the pores whereas the larger ones, cannot and wil elute first from the column.

Answer 10

Size exclusion

Question 11

: molecule separation or removal from another

Answer 11

Elution

Question 12

• designed for purification; a column constructed of stainless steel or glass.

Answer 12

column

Question 13

, the stationary phase is a thin film of liquid that is fixed onto small granular particles packed into the column

Answer 13

Packed

Question 14

: can be made narrower than packed columns because their stationary liquid phase is actually coated as a very thin film directly onto the column’s inner wall.

Answer 14

Capillary

Question 15

: the time ti takes for a compound to elute. This value is characteristic of a compound, and is related ot the strength of its interaction with the stationary and mobile phase; term to determine a compound’s identity.

Answer 15

Retention time (Tr)

Question 16

a phenomenon based on the electronegativity difference between two atoms.

Answer 16

Polarity

Question 17

• phenomenon of nonpolar (hydrophobic molecules) ot not dissolving water (e.g oil and waxes)

Answer 17

Hydrophobicity

Question 18

(acts as mobile phase)-

Answer 18

Gas cylinder

Question 19

: Helium, Hydrogen, Nitrogen

Answer 19

Must be chemically inert

Question 20

• samples are injected using an automated syringe pipet system; a plastic septum (injection port) si heated very high temperatures.

Answer 20

Sample injector