Chromatography

Question 1

What is the end result of chromatography called

Answer 1

Chromatogram

Question 2

Why must the spot of indicator be above the water level

Answer 2

So the indicator does not dissolve in water

Question 3

Why must the starting line be drawn with a pencil not a pen

Answer 3

Dyes from the pen ink will result in additional separated components introduced into the chromatogram

Question 4

How to identify colour less substances (for example amino acids)

Answer 4

Spray/add locating agents such as (ninhydrin) onto the chromatogram, which reacts with (amino acids) to form coloured products, thus making them visible

Question 5

whats retention factor, how do u calculate it

Answer 5

divide the distance travelled by the component by the distance travelled by the solvent. (the answer is always less than 1)
lets say the solvent travelled 6cm and the component only travelled 3cm so 3/6 = 0.50 retention factor

Question 6

when doing 2d chromatography, y does the first one have less spots

Answer 6

this is because they have the same solubility in solvent x and will have the same retention factor