Chromatography 1 Flashcards
Isocratic elution
The composition of the mobile phase is kept constant
Gradient elution
Composition of the mobile phase is varied
Stop-flow injection
The solutions are forced from syringes into a mixing chamber where they are mixed rapidly
Reverse-phase packing
Stationary phase of the chromatography is hydrophobic
Normal-phase packing
The stationary phase is polar and he mobile phase is nonpolar
Ion-pairing chromatography
A type of column chromatography where ions in solution can be paired or neutralized and separated as an ion pair in a reverse-phase column
Usually ionic compounds that contain hydrocarbon chains
Ion chromatography
Chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger/stationary phase
Reverse phase packing
Bulk property detector
Compares an overall change in physical properties of the mobile phase with and without analyte
Measures some bulk physical property of the fluent
Solute property detector
Responds to the physical properties of the solute not shown in the mobile phase
Measure physical or chemical property unique to the solute
Sparging
Technique which involves bubbling a chemically inert gas, such as nitrogen, argon, or helium through a liquid
Can be used to remove dissolved gases from a liquid
Retention factor (K) (Capacity)
Time required to elite our peak (Tr) - the time for the mobile phase (Tm)
Selectivity factor (alpha symbol)
Based on stationary phase and its selectivity for the mobile phase and things in the mobile phase
Efficiency (N^(1/2)/4)
N = # if theoretical plates
The more theoretical plates the more efficient the column
Master Resolution Equation
Capacity x Selectivity x Efficiency
How to change Capacity
Gas chromatography
Change the temperature
(Lowering temp by 25 degrees C doubles k)
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