Chromatographic Techniques Flashcards
What spray reagents are used to detect Flavonoids/Steroids
Antimony (III) Chloride
Aluminum Chloride
Positive result for flavonoids/steroids
intense yellow to orange visible zones
fluorescent color under 365 nm
What spray reagents are used to detect Phenols, Tannins, Flavonoids
Potassium ferricyanide-Ferric chloride
Positive result for phenols, tannins, flavonoids
blue spots
What spray reagents are used to detect Alkaloids
Dragendorff’s reagent
Positive result for alkaloids
brown orange visible spots
What spray reagents are used to detect Cardenolides
3,5-dinitrobenzoic acid (Kedde reagent)
positive result for cardenolides
blue to red-violet colored zones
What spray reagents are used to detect Indoles?
Van-Urk Salkowski Test
Positive result for Indoles
Blue-violet spots
What spray reagents are used to detect Anthraquinones, Anthrones, Coumarins?
Methanolic KOH (Borntrager Reagent)
Positive result for Anthraquinones, Anthrones, Coumarins?
Anthraquinones : orange color
Anthrones : yellow color at 365 nm
Coumarins : Blue color at 365 nm
What spray reagents are used to detect sugars?
a-naphthol-sulfuric acid (corrosive)
Positive result for sugar
Blue spots
What spray reagents are used to detect Steroids, essential oils?
Vanillin-sulfuric acid (corrosive)
