CHP 8: B Cell Development Flashcards
Where do B-1 B cells reside?
Body cavities
What is the most abundant subtype of B cell?
B-2 B cells
What is the function of the B-2 B cells?
survey and combat infections after activation in the secondary lymphoid tissues
Which B cells are found in the spleen and protect against bloodborne pathogens?
Marginal zone B cells
The immunoglobulin IgG is typically found in?
Serum/Bloodstream
The immunoglobulin IgA is typically found? Provide examples
Mucosal surfaces such as the respiratory, digestive, and urinary tract
Which immunoglobulin is known for first line of defense from the adaptive immune system?
IgM
B-1 B cells secrete which type(s) of immunoglobulin after maturation and differentiation?
IgM
B-2 B cells secrete which type(s) of immunoglobulins after maturation and differentiation?
All isotypes
Marginal zone B cells secrete which type(s) of immunoglobulins after maturation and differentiation?
IgM; IgG
Describe the cell lineage from HSC’s to Lymphoid progenitor cells
1) Hematopoietic stem cells (HSC’s)
2) Multipotent progenitor cells
3) Lymphoid primed multipotent progenitor cells
4) Lymphoid progenitor cells
What causes HSCs to differentiate to multipotent progenitor cells?
c-Kit (expressed on HSCs surface) binding to signaling molecule stem cell factor on stromal cells?
Which cells play a major role in driving HSCs differentiation into multipotent progenitor cells?
Stromal cells
Which unique molecules do multipotent progenitor cells begin to express in order for them to differentiate into ____________?
CD34; CXCR4
Lymphoid primed progenitor multipotent cells
Which cytokine plays a role in promoting B cell differentiation?
IL-7
Which cells secrete IL-7 to promote B cell differentiation?
Bone marrow stromal cells
Cells expressing major components of _________ _____________ are destined to become lymphocytes because they are the only once that use this recombinase
V(D)J recombinase
Binding of IL-7 promotes ___________ remodeling, allowing access to the immunoglobulin genes to the ___________ _____________.
chromatin; VDJ recombinase
Lymphoid primed multipotent progenitor cells begin to express ______, ______, and _______ by the end of their development and become ________________.
RAG1;RAG2; TdT
Common lymphoid progenitor cells are able to differentiate into which cells?
1) NK cells
2) innate lymphoid cells
3) B cells
4) T cells
B cells begin development in the _______ (near the surface of the bone)
Endosteum
Name the development stages of B cells in the bone marrow
1) Early pro B cells
2) Pro B cells
3) Pre B cells
4) Immature B cells
What are the checkpoints in B cell development?
1) checkpoint of the heavy chain rearrangement events
2) checkpoint of the light chain rearrangement events
What are the two major functions of the spleen?
1) Filter and cleanse the blood
2) Lymphocyte proliferation and + and - selection of B cells
__________ B cells migrate from the bone marrow to the spleen
Transitional
T1 transitional B cells undergo (+/-) selection to select cells with high affinity to self antigen are removed from the population
Negative selection
Early pro B cells have which receptor on their membrane in order to differentiate into a late pro B cell?
IL-7R
Through which stage of b cell development is the heavy chain test for functionality?
Late pro B cell
Which segments are recombined first of the heavy chain?
D and J
