chloroplasts Flashcards

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1
Q

state the 4 layers of the chloroplast from outer to innermost

A
  • outer membrane
  • inner membrane space
  • inner membrane
  • stroma
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2
Q

what are granum

A

stacked thylakoids

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3
Q

what are thylakoids

A

folded membranes containing photosynthetic pigments involved in light dependent reactions

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4
Q

what is the stroma

A

fluid interior containing enzymes involved in the light independent reaction

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5
Q

what are the 2 primary pigments

A

chlorophyll a and b

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6
Q

what is an example of an accessory pigment

A

carotenoids

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7
Q

chlorophyll a absorbs which colours?

A

yellow, green

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8
Q

chlorophyll b absorbs which colours

A

blue, green

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9
Q

carotenoids absorb which colour

A

orange

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10
Q

where are pigments located

A

embedded in thylakoid membrane

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11
Q

what do the pigments do?

A

form light harvesting systems - each pigment absorbs a different wavelength of light

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12
Q

summarise the process of separating pigments using TLC

A
  • crush leaf and grind with sand & propanone to extract pigments
  • transfer to watch glass & dry to evaporate moisture
  • add propanone to absorb pigments
  • add spot to TLC paper and place tip in running solvent
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13
Q
A
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