ChemPath: Potassium Flashcards
What is the normal range for serum potassium?
3.5-5.0 mmol/L
What are the two main hormones involved in the regulation of potassium?
- Angiotensin II
- Aldosterone
Outline how the renin-angiotensin-aldosterone system works.
- Reduced perfusion or low sodium will stimulate the production of renin from the juxta-glomerular cells
- This cleaves angiotensinogen to angiotensin I
- This is then converted by ACE in the lungs to angiotensin II → stimulates aldosterone release from the adrenals
- Aldosterone stimulates sodium reabsorption and potassium excretion in the principal cells of the cortical collecting tubule
NOTE: water will also be drawn in with the sodium so aldosterone should not greatly affect sodium concentration
Where aldosterone acts
Principal cortical cells
collecting duct
Outline the mechanisms of action of aldosterone.
- Aldosterone binds to MR and stimulates the transcription of ENaC channels
- Aldosterone binding to MR also leads to increased Sgk1 which inhibits Nedd4
- Nedd4 usually ubiquitinates sodium channels and degrades them
- Inhibition of Nedd4 leads to preservation of sodium channels thereby increasing sodium reabsorption
- As you reabsorb more sodium, the lumen becomes more negative and K+ will move down the electrochemical gradient into the lumen via ROMK channels
How Na affects K excretion
Sodium resorbed - making the lumen more negative
K then excreted along the electrochemical gradient
What are the main stimuli for aldosterone release?
- Angiotensin II
- High potassium
List some causes of hyperkalaemia.
- Reduced GFR (renal failure)
- Reduced renin activity (renal tubular acidosis type 4, NSAIDs)
- ACE inhibitors/ ARBs (losartan)
- Addison’s disease
- Aldosterone antagonists (spirinolactone / epleronone)
- Potassium release from cells (rhabdomyolysis, acidosis)
Causes of hyperkalaemia if someone asks
- Renal failure (reduced Excretion)
- Drugs (ACEi / Angiotensin / Aldosterone antagonists)
- Low Aldosterone
- Addisons
- Type 4 renal tubular acidosis (low renin, low aldosterone)
- Release from cells (Rhabdomyolysis, acidosis)
Explain how acidosis leads to hyperkalaemia.
- When plasma H+ concentration is high, the cells try to take in more H+ from the plasma
- To maintain electrochemical neutrality, K+ must leave the cell when H+ enters
- This leads to hyperkalaemia
Outline the management of hyperkalaemia.
- 10 ml 10% calcium gluconate
- 50 ml 50% dextrose + 10 U insulin
- In reality - 100ml 20% dextrose
- Nebulised salbutamol - beta agonists drive K into cell
- Treat the cause
List some causes of hypokalaemia.
- GI loss (diarrhoea)
- Renal loss
- Hyperaldosteronism, Cushing’s syndrome (binding to MR)
- Increased sodium delivery to distal nephron
- Osmotic diuresis
- Redistribution into cells
- Insulins
- Beta-agonists
- Alkalosis
- Rare causes
- Renal tubular acidosis (type 1 and 2)
- Hypomagnesaemia
Describe Renal K handling
More Na to distal tubules causes increased exchange of K
Block of Na/CL channels by Thiazides and Gitelman’s syndrome
Name two conditions that can block the triple transporter.
- Loop diuretics
- Bartter syndrome (mutation in triple transporter)
Name two conditions that can block the Na+/Cl- cotransporter.
- Thiazide diuretics
- Gitelman syndrome (mutation in Na+/Cl- cotransporter)