Chemistry Experiment 6 Flashcards
Estimation of vit-C is done by which method ?
Iodometric Method
Vitamin C also called as -
Ascorbic acid
Vitamin C is oxidized to do what ?
To dehydroascorbic acid by iodine
Aim of Estimation of Vit-C ?
To determine the amount of Vit-C sample volumetrically by Iodometric method.
How is the concentration of Vit-C determined ?
The excess of iodine left behind is back titrated with standard sodium thiosulphate solution from the amount of iodine consumed, the concentration of Vit-C can be determined.
What do Vit-C sample make in with which acid to prevent ?
Vit-C sample make in 4 % oxalic acid to prevent the reduction back to the ascorbic acid.
_________+ I2 ——-> ___________
Ascorbic acid + I2 ——-> Dehydroascorbic acid
Requirements of Estimation of Vit-C ?
________+ I2 —(excess KI)—> ________+________
Na2S2O3 +I2 —(excess KI)–> Na2S2O3 + 2NaI
Indicator of Estimation of Vit-C
Starch
End point of Estimation of Vit-C
Blue to colorless
Solution in burette
Na2S2O3
Solution in flask
20 ml of 0.1 NI2
Difference in standardization of I2 AND Estimation of Vit-C ?
Everything is same except that in estimation the prepared solution is kept in dark for 5 min and then starch is added & in standardization the starch is added early.