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Biochem > Chemical Tests for Proteins > Flashcards

Chemical Tests for Proteins Flashcards

1
Q

Complete Hydrolysis

What amino acids are partially destructed in acid hydrolysis

A
  • cys
  • tyr
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2
Q

Complete Hydrolysis

What amino acid are completely destructed in acid hydrolysis

A
  • trp
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3
Q

Complete Hydrolysis

What amino acids are incompletely liberated in acids hydrolysis

A
  • val
  • ile
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4
Q

Complete Hydrolysis

What amino acids are destructed and racemized in acid hydrolysis

A
  • ser
  • thr
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5
Q

Complete Hydrolysis

What amino acids are converted to asp and glu in acid hydrolysis

A
  • asn
  • gln
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6
Q

Complete Hydrolysis

What amino acid is preserved or not destroyed in basic hydrolysis

A
  • trp
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7
Q

Complete Hydrolysis

What amino acids are completely destructed in basic hydrolysis

A
  • gln
  • asn
  • arg
  • ser
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8
Q

Qualitative Tests for Proteins

What test detects the presence of peptide bonds

A

Biuret test

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9
Q

Qualitative Tests for Proteins

What test has a positive result of pink to violet to blue coloration

A

Biuret test

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10
Q

Qualitative Tests for Proteins

What test has this principle: Complexation of Cu2+ with a peptide bond

A

Biuret test

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11
Q

Qualitative Tests for Proteins

What test detects the free α-amino acids and amines

A

Ninhydrin test

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12
Q

Qualitative Tests for Proteins

What test has the reagents: NaOH and CuSO4

A

Biuret test

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13
Q

Qualitative Tests for Proteins

What test has the reagents:
1,2,3-indanetrione monohydrate and EtOH

A

Ninhydrin test

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14
Q

Qualitative Tests for Proteins

What test has a positive result of:
- Blue or blue-purple color
- yellow-orange (Proline)

A

Ninhydrin test

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15
Q

Qualitative Tests for Proteins

What test has this principle: oxidative decarboxylation and deamination followed by condensation

A

Ninhydrin test

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16
Q

Qualitative Tests for Proteins

What test detects aromatic amino acids (Y, W, F)

A

Xanthoproteic test

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17
Q

Qualitative Tests for Proteins

What test has this principle: nitration of aromatic ring

A

Xanthoproteic test

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18
Q

Qualitative Tests for Proteins

What test has the reagents: conc. HNO3 & conc. NaOH

A

Xanthoproteic test

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19
Q

Qualitative Tests for Proteins

What test has a positive result of:
- yellow solution (heating)
- orange sol’n (excess NaOH)

A

Xanthoproteic test

20
Q

Qualitative Tests for Proteins

What test detects phenolic ring (Y)

A

Millon’s test

21
Q

Qualitative Tests for Proteins

What test has a positive result of: old rose/flesh, purple-red ppt

A

Millon’s test

22
Q

Qualitative Tests for Proteins

What test has the reagents: HgSO4 in H2SO4

Hg (Mercury)

A

Millon’s test

23
Q

Qualitative Tests for Proteins

What test has this principle: complexation (mercuration & nitration or nitrosation) and complexation of nitrohydroxylphenyl derivatives qith Hg2+

A

Millon’s test

24
Q

Qualitative Tests for Proteins

What test has this principle: reduction of oxalate to glycolate and acid-catalyzed condensation of 2 trp’s with glyoxylic acid

A

Hopkins-Cole test

25
Q

Qualitative Tests for Proteins

What test has the reagents: Glyoxylic acid and conc. H2SO4

A

Hopkins-Cole test

26
Q

Qualitative Tests for Proteins

What test has a positive result of: pink to violet interface / ring in test tube

A

Hopkins-Cole test

27
Q

Qualitative Tests for Proteins

What test detects indole group in Trypthophan (trp)

A

Hopkins-Cole test

28
Q

Qualitative Tests for Proteins

What test detects guanidino/guanidine group of Arginine (arg)

A

Sakaguchi test

29
Q

Qualitative Tests for Proteins

What test has a positive result of: Red to red-orange color

A

Sakaguchi test

30
Q

Qualitative Tests for Proteins

What test has the reagents: α-napthol, NaOBr, NaOH, Urea

A

Sakaguchi test

31
Q

Qualitative Tests for Proteins

What test has this principle: base-catalyzed of α-napthol (OA) with guanidino group

A

Sakaguchi test

32
Q

Qualitative Tests for Proteins

What test has this principle: degradation and substitution reaction to form PbS

A

Fohl’s/Lead Acetate test

33
Q

Qualitative Tests for Proteins

What test has the reagents: Lead(II) acetate and NaOH

A

Fohl’s/Lead Acetate test

34
Q

Qualitative Tests for Proteins

What test has a positive result of: Brown or black ppt

A

Fohl’s/Lead Acetate test

35
Q

Qualitative Tests for Proteins

What test detects S-containing amino acids (M & C)

A

Fohl’s/Lead Acetate test

36
Q

Qualitative Tests for Proteins

What test detects presence of His and Tyr

A

Pauly’s test/Diazo reaction

37
Q

Qualitative Tests for Proteins

What test has a positive result of: Red color

A

Pauly’s test/Diazo reaction

38
Q

Qualitative Tests for Proteins

What test has the reagents: Sulfanic acid, NaNO2, Na2CO2

A

Pauly’s test/Diazo reaction

39
Q

Qualitative Tests for Proteins

What test has this principle: Diazotized sulfanilic acid coupled with amino phenol (forms a color red azo compound in cold)

A

Pauly’s test/Diazo reaction

40
Q

Qualitative Tests for Proteins

What test has this principle: Complexation

A

Nitroprusside Test

41
Q

Qualitative Tests for Proteins

What test has the reagents: Na2Fe(CN)5NO in dilute NH3

A

Nitroprusside Test

42
Q

Qualitative Tests for Proteins

What test detects presence of -SH group (C)

A

Nitroprusside Test

43
Q

Qualitative Tests for Proteins

What test has a positive result of: red color

A

Nitroprusside Test

44
Q

Qualitative Tests for Proteins

What test has a positive result of: red litmus paper to blue (basic)

A

Amide test

45
Q

Qualitative Tests for Proteins

What test detects 1°,2°,3° amides and nitriles

A

Amide test

46
Q

Qualitative Tests for Proteins

What test has the reagent/s: NaOH

A

Amide test

47
Q

Qualitative Tests for Proteins

What test has this principle: Base-catalyzed hydrolysis

A

Amide test

Biochem (1 decks)

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