Chart Flashcards
1
Q
BrightField Cells Stained image How does the object and Background appear? What cell structure can be seen?
A
Dead Stained 2D Dark objects are visible against bright background None
2
Q
Dark Field Illumination Cells Stained image How does the object and background appear? What cell structure can be seen?
A
Live None 2D Light objects are visible against dark background Cilia and Flagella
3
Q
Phase Contrast Cells Stained image How does the object and background appear? What cell Structure can be seen?
A
Live None 2D None Cilia and Flagella clearly than darkfield
4
Q
Differential Interference Contrast Cells Stained image How does the object and background appear? What cell Structure can be seen? Use/Resolution
A
Live
None
3D
None
Cilia and Flagella better than darkfield/phase contrast
Best Resolution
5
Q
Fluorescence Cells Stained image How does the object and background appear? What cell Structure can be seen? Use/Resolution
A
Dead
Fluorescent dye creates visible light
2D
None
None
Quick diagnosis of TB/Syphilis
6
Q
Transmission Electron Cells Stained image How does the object and background appear? What cell Structure can be seen? Use/Resolution
A
Dead
Heavy metal salt
2D
None
Organelles
Best resolution of all
7
Q
Scanning Electron Cells Stained image How does the object and background appear? What cell Structure can be seen? Use/Resolution
A
Dead
None
3D
None
Surface view only
None
8
Q
Scanning Probe Cells Stained image How does the object and background appear? What cell Structure can be seen? Use/Resolution
A
Specimen is scanned, image is sent to comp.
None
None
None
None
Two Drawbacks
1. Slower in acquiring images
2. Max images size is smaller.
