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Microbio Lab Practical > Characteristic Tests > Flashcards

Characteristic Tests Flashcards

1
Q

Glucose: color change

A

Red to Yellow if fermentation is present

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2
Q

Glucose: pH indicator

A

Phenol Red

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3
Q

Glucose Fermentation Process

A

(1) Fermentation positive

(2) They release acidic byproducts (3) Leading to a color change

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4
Q

Lactose: color change

A

Red to Yellow if fermentation is present

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5
Q

Lactose: pH indicator

A

Phenol Red

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6
Q

Lactose Fermentation Process

A

(1) Fermentation (+)
(2) Acidic byproduct
(3) Color change

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7
Q

Sucrose: color change

A

Red to Yellow

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8
Q

Sucrose: pH indicator

A

Phenol red

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9
Q

Sucrose: Fermentation Process

A

(1) Fermentation (+)
(2) Acidic by product
(3) Color change

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10
Q

Mannitol: color change

A

Red to Yellow

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11
Q

Mannitol: pH indicator

A

Phenol red

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12
Q

Mannitol: Fermentation Process

A

(1) Fermentation (+)
(2) Acidic by product
(3) Color change

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13
Q

Tryptone: color change

A

Clear to Clear with pink ring

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14
Q

Tyrptone: Reagent

A

Kovacs reagent

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15
Q

Tryptone: Production pathway

A

Tryptophan (AA) in media breaks down into indole and pyruvic acid

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16
Q

MR-VP: color change

A

Clear to clear/growth

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17
Q

MR: what type of pathway

A

Mixed Acid Fermentation Pathway

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18
Q

MR: test for positive and negative

A

(1) Straight line of diffused color = positive

(2) Diffused color = negative

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19
Q

VP: what type of test

A

2,3 butanediol production pathway

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20
Q

Urea: color change

A

Yellow/orange to light/hot pink

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21
Q

Urea: Purpose

A

Check for urease enzyme

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22
Q

Urea: pH indicator

A

Phenol Red

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23
Q

Urea: production pathway

A

(1) Urea
(2) To ammonia
(3) pH increases
(4) Turns pink in color

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24
Q

Simmons citrate: color

A

(1) Green to blue

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25
Q

Simmons citrate: Purpose

A

To see if organism can use citrate for growth/ metabolism

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26
Q

Simmons citrate: pH indicator

A

Bromothymol blue

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27
Q

Simmons citrate: Production pathway

A

Citrate to Pyruvic acid

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28
Q

Phenylalanine slant: color change

A

Clear to Green

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29
Q

Phenylalanine slant: Purpose

A

Check for presence of phenylalanine deaminase enzyme

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30
Q

Phenylalanine slant: Reagent

A

Ferric chloride

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31
Q

Phenylalanine slant: Production pathway

A

(1) Phenyl pyruvic acid and ammonia by product from Phenylalanine AA

32
Q

Kligers Iron: color change

A

Red to Red with a black butt

33
Q

Kligers Iron: Purpose

A

Check for presence of Cysteine desulfurase enzyme

34
Q

Kligers Iron: Production pathway

A

Cysteine to H2S and Iron leading to a black butt which is anaerobic

35
Q

Semi solid agar: color change

A

Clear to clear with red streak line

(1) colorless in oxidized state (2) red in reduced state

36
Q

Semi solid agar: Purpose

A

Check for motility

37
Q

Semi Solid Agar: Checking for what presence

38
Q

Nutrient Agar: color

A

Clear to clear with colony growths

39
Q

Eosin methylene blue

A

(1) Purple/red plate to purpleish pink colony growths (2) Green if colony growths/ fermentation is positive (3) Checks for fermentation of lactose (4) pH sensitive dye is methylene blue (5) Selective for gram (-) organisms

40
Q

Mannitol Salt Agar Plate

A

(1) Check for mannitol fermentation (2) pH sensitive dye: phenol red (3) If fermentation is positive the plate turns yellow, if not it turns pink (4) Selects for organisms that can handle high salt concentrations

41
Q

Blood Agar Plate

A

Used for throat culture because the blood agar breaks down hemoglobin to get iron

42
Q

TSA Plate

A

Universal Plate

43
Q

Partial Lysis Blood cells

A

Streptococcal pneumoniae (green pigment)

44
Q

Complete Lysis Blood cells

A

Streptococcal pyrogenes (clear spots)

45
Q

No lysis Blood cells

A

Enterobacter faecalis

46
Q

MH plate

A

Mueller Hinton, Universal Plate

47
Q

Brewers Plate

A

Contains sodium thioglycollate

48
Q

Resazurin

A

Oxygen indicator in FTG tube and Brewers plate

49
Q

Agar Deep

A

Check to see if organism is anaerobic, aerobic, facultative anaerobic, aero-tolerant anaerobe

50
Q

FTG Tube

A

(1) Fluid thioglycollate, contains sodium thioglycollate, converts O2 to H2O (2) Favors anaerobic

51
Q

Facultative Anaerobic

A

Favors aerobic conditions but will still grow anaerobically

52
Q

Obligate Anaerobe

A

Only grows without oxygen

53
Q

Aerobe

A

Only grows with oxygen

54
Q

Aerotolerant Anaerobe

A

Grows with out without oxygen evenly

55
Q

UV rays

A

Forms pyrimidine dimers to kill organisms (1) 260 nm is most germicidal effect (2) 4nm UV (3) 800 nm is infrared

56
Q

Quadrant streaking

A

Check for contamination

57
Q

Grams Iodine and Crystal violet

A

Forms an insoluble complex in the PG layer in gram +

58
Q

Catalase

A

(1) Purpose: catalase (2) Hydrogen peroxide to H2O and O2 (3) Bubbles

59
Q

Gram positive characterisitics

A

Purple, clustered

60
Q

Gram negative characteristics

A

Pink, linked

61
Q

Sabouraud Agar

A

Low pH so good for skin growing organisms

62
Q

Antiseptic

A

can be used on living tissue

63
Q

Disinfectant

A

can be used on inanimate objects

64
Q

Antigen

A

A foreign substance that enters the body and mounts an immune response

65
Q

Antigen binding site

A

Protein sequence, receptor, epitope

66
Q

Antibody

A

Response of human immune system to any foreign substance is to produce anti-
antigen, antibody it is also referred to as immune response

67
Q

Immunity Innate vs Acquired

A

(1) Innate is born with it its natural (2) Acquired is from mother or from vaccine

68
Q

Type A

A

Antibody B, Antigen A, Receives from A and O

69
Q

Type B

A

Antibody A, Antigen B, Receives from B and O

70
Q

Type AB

A

Antibody none, Antigen AB, Receives from A,B,AB,O

71
Q

Type O

A

Antibody A and B, Antigen: none, Receives from O

72
Q

IgG

A

Passes through placenta

73
Q

IgM

A

Cannot pass through placenta

74
Q

Rh Factor

A

Rh present = positive (Antigen Rh +, Antibody Rh -) , Rh not present = negative ( Antigen Rh - Antibody Rh +)

75
Q

No clump

76
Q

Clump

77
Q

ELISA

A

Enzyme Linked Immuno Sorbant Assay

Microbio Lab Practical (1 decks)

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