Chapter9 -- Negative Stain

Question 1

Indirect Simple Stain

Answer 1

acidic chromogen, or negatively charged chromophore – which is repelled by the bacterial cell and background becomes stained EX: negative stain

Question 2

eosin and nigrosin

Answer 2

Examples of Negative Stains

Question 3

To Know:

Answer 3

• negative stains slides are not heat fixed so cell shape and size are not distorted
• negative stains are appropriate for visualizinf capsules, spirilla and spirochetes
• place a drop on one end of slide and use a clean slide to spread over sample slide

Question 4

Treponema pallidum

Answer 4

syphilis