Chapter One Flashcards
How are cells different?
Shape, Size, and Function;
Diverse Chemical needs/activities
Flow of Genetic Information
All living cells share; Genetic instruction stored in DNA (genes code for proteins/4 nucleotides)…Transcribed into RNA… Then transcribed into proteins(20 amino acids).
Viruses
Require a live host to replicate; Uses the host’s replication/transcription/protein synthesis machinery
Microscope
Invented in the 17th Century
Types of Microscopes used in Biology
Light
Transmission electron
Scanning electron
Schleiden/Schwann/Virchow
Discovered cells are the basic living unit of function, all organisms are composed of one or more cells, and all cells come from division of existing cells
Louis Pasteur
Confirmed ideas of Schwann, Schleiden, and Virchow by observing plant cells for 2.5 hours
Steps for preparing tissue samples for microscopy imaging.
Fixation, Embedding, Sectioning, and Staining
Fixation
Preserve tissue in native state
Embedding
Replacing the water in a cell with wax to support the tissue
Sectioning
Creating thin slices of the specimen
Staining
Making contrast
SEM
Scanning electron microscope; Visualizes surface structures; Uses magnets and not lenses; reveals 3-D images
TEM
Transmission electron microscope; visualizes internal structures…nanometer range; no 3-D imaging
Light microscopes
Cannot see smaller cell components like organelles