Chapter II Cleaning and sterilization

Question 1

required in any laboratory handling bio-hazardous agents. It is important to reduce the risk of community-acquired disease (e.g., common cold, flu, staphylococcus, etc.)

Answer 1

Regular disinfection

Question 2

reducing risk of contact exposure to infectious agents through _____________ is an important part of regular laboratory maintenance to effectively inactivate infectious agent.

Answer 2

routine cleaning, and disinfection

Question 3

Refers to the removal of dirt and impurities, including germs, from surfaces. Doing this alone does not kill germs. But by removing the germs, it decreases their number
and therefore any risk of spreading infection. Can be achieved with soap and water.

Answer 3

Cleaning

Question 4

works by using chemicals to destroy, inhibit growth, or removal microbes on surfaces. But killing germs remaining on a surface after cleaning further reduces any risk of spreading infection.

Answer 4

Disinfecting

Question 5

means the complete destruction of all the micro-organisms including
spores, from an object or environment.

Answer 5

Sterilization

Question 6

two types of sterilization commonly used in our laboratory

Answer 6

dry heat sterilization and moist heat sterilization

Question 7

using the principle of steam under pressure through autoclave or pressure cooker

Answer 7

Moist heat sterilization

Question 8

the simple method practiced usually against vegetative form of bacteria.

Answer 8

Boiling at t 100℃

Question 9

used to keep the autoclaved media at around 45-50℃ before dispensing it to the Petri dish.

Answer 9

Water bath

Question 10

important in that it does not affect the glass surface; do not corrode metals, able to sterilize powders, oil and other viscous substances without getting moist or evaporated.

Answer 10

Dry heat sterilization

Question 11

used to fix the smears like in M. tuberculosis and to reduce air contamination at mouth of broth tube during sub culture, or sterilize inoculating loops)

Answer 11

Flaming

Question 12

uses a very high temperature (160-180℃) for one hour.

Answer 12

Hot air oven

Question 13

first by tap water, soap, savlone and then other detergent

Answer 13

cleaning fir equipments

Question 14

cleaning for wounds

Answer 14

antiseptics like phenols