Chapter 9 - Principles of Serological Testing

Question 1

Blood is collected aseptically by venipuncture into a sterile tube that does not contain an anticoagulant

Answer 1

Red top, Gold top, or Serum separator tube

Question 2

Care must be taken to avoid hemolysis, as this may produce a _______

Answer 2

false-positive test

Question 3

The blood specimen is allowed to clot at _________ or at ____, depending upon the protocol for the specific procedure.

Answer 3

room temperature or at 4°C

Question 4

(T/F)
Fresh serum that has not been inactivated by heating is usually recommended for testing.

Answer 4

true

Question 5

(T/F)
complement must be inactivated because it
interferes with test results.

Answer 5

true

Question 6

The serum is heated to _____ for _______ to destroy complement proteins.

Answer 6

56°C for 30 minutes

Question 7

In either circumstance, if testing cannot be performed immediately, serum may be stored between ___ and ___ for up to ______

Answer 7

between 2°C and 8°C for up to 72 hours

Question 8

If there is any additional delay in testing, the serum should be frozen at _____ or colder.

Answer 8

–20°C

Question 9

commonly used to measure either serum for testing or liquid for making reagents and dilutions.

Answer 9

pipettes

Question 10

(T/F)
The pipettes are calibrated to transfer or
deliver specific volumes as marked on their surfaces.

Answer 10

true

Question 11

pipettes are categorized as either ______ or ______

Answer 11

volumetric or graduated

Question 12

These are marked and calibrated to deliver only one volume of a specified liquid.

Answer 12

volumetric pipettes

Question 13

These pipettes enable the user to dispense the
exact measure of liquid with a small drop left behind.

Answer 13

volumetric pipettes

Question 14

Pipettes are usually labeled “TD,” meaning _______

Answer 14

to deliver

Question 15

It has an oval bulb in the center and a tapered dispensing end

Answer 15

volumetric pipettes