Chapter 9 Flashcards
Short tandem repeats (STRs)
Are useful in identifying specific individuals.
Mitochondrial DNA (mtDNA) in humans
Can be used to identify related people.
DNA molecules that contain pieces of DNA from two different sources are defined as
Recombinant DNA
Common vectors used for cloning genes are
Viruses AND plasmids
The molecules used to cut target DNA in genetic engineering are
Restriction enzymes
Identify the statement about digestion of DNA by restriction enzymes that is not true
One strand of the DNA molecule is cut.
Restriction enzymes have proved so useful in manipulating DNA because
They cut at defined sites and the sticky ends make it very easy to allow recombination of any type of DNA
The molecule(s) that act ad molecular glue to bind DNA fragments together is/are
DNA ligase
Agarose gel electrophoresis separates nucleic acid fragments according to
Size
A dye often used for its ease and sensitivity to visualize nucleic acid after agarose gel electrophoresis is
Ethidium bromide
The energy to separate fragments during agarose gel electrophoresis is supplied by
Electricity
The agarose used in electrophoresis
Separates DNA fragments by size
The gene for human insulin has been cloned in
E. Coli.
Genetic engineering
Allows the use of bacteria as production factories for a number of molecules and relies on recombinant DNA technology
Genetic engineering of plants has so far produced
All of the answer choices are correct
Pest-resistant plants
Plants with increased nutritional value
Plants that are herbicide resistant
The entire set of cloned fragments of the complete human genome is termed a
DNA library
Which of the following is NOT true about an ideal vector
A vector contains an RNA primer
Host cells containing recombinant DNA can be selected on the basis of the properties of the
Vector
