Chapter 8 - Venipuncture Equipment Flashcards

1
Q

Anticoagulants

A

Acid Citrate Dextrose (ACD)

Citrate-Phosphate-Dextrose (CPO)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Coagulation Preventives

A

Oxalates
Citrates
EDTAs
Heparin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Oxalates, Citrates, and EDTAs prevent coagulation by…

A

removing calcium and forming insoluble calcium salts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Which coagulation preventatives should not be used for a calcium study?

A

Oxalates, Citrates, and EDTAs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Citrates are frequently used for what kind of studies?

A

Coagulation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What coagulation preventative is best for platelet counts and platelet functions tests? Why?

A

EDTAs because it prevents platelet aggregation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What coagulation preventative is best for blood films? Why?

A

EDTAs because it has minimal distortion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Heparin prevents clotting by…

A

inactivating the blood clotting chemicals (thrombin and factor X)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What study is ordered when septicemia is suspected?

A

Blood cultures

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Blood Cultures must be completely…

A

STERILE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

SPS

A

Sodium Polyanethol Sulfonate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

EDTA

A

Ethylendiamine Tetra-Acetic Acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Heparin is a

A

Mucopolsaccharide that prevents clotting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Phagocytosis

A

defense mechanism to kill microorganisms in blood

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Why is SPS used in blood cultures?

A

Inhibits phagocytosis thus enhancing chances of detecting bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Yellow Tubes with SPS are for

A

Blood Cultures

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Yellow Tubes with ACD are for

A

Preserving blood for donation, specialty blood banking (like HLA typing and DNA testing)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

HLA

A

Human Leukocyte Antigen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

HLAs are studied for

A

Paternity Evaluation and donor compatibility

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Two Types of Yellow tubes with SPS

A

Unplugged Venting Unit for aerobic incubation

Plugged Venting Unit for anaerobic incubation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

For aerobic incubation you should use a Yellow Tube with SPS with

A

Unplugged Venting Unit

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

For anaerobic incubation you should use a Yellow Tube with SPS with

A

Plugged Venting Unit

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

TDM

A

Therapeutic Drug Monitoring

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Gold Inversion #

A

8

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Yellow with SPS Inversion #
8
26
Light Blue Inversion #
3-4
27
Let gold tubes sit _____ before centrifuge
30 minutes
28
During centrifugation of Gold tubes...
polymer barrier moves upward to the serum-clot interface | Separates serum from fibrin and cells
29
RST
Rapid Serum Tubes
30
Orange and Grey/Yellow Tubes are
Rapid Serum Tubes
31
Orange Tubes Additive
Thrombin
32
Thrombin additives will clot blood in
less than 5 minutes
33
Red additive
None
34
Red tubes are used for collection of
serum
35
Blood collected in Red Tubes ______ clot.
WILL
36
How long does it take a fibrin clot to form in a red tube?
30 minutes
37
If you centrifuge a red tube what will happen?
Serum will separate from the blood
38
Clear/White Additive
None
39
Clear/White Tubes are used for
Discard or Secondary Specimen collection
40
Green/Mint Green/Gray and Green additives
Anticoagulants Sodium Heparin, Annomium Heparin, and Lithium Heparin
41
Green/Mint Green tubes are used for any test requiring
plasma or whole blood
42
Why is the green tube good for a STAT test?
The whole blood can be tested without clot formation or centrifuge
43
Why is heparinized plasma or whole blood better for potassium testing rather than the serum in red tubes?
Serum in red tubes can contain sporadic increased potassium levels as a result of the potassium released by the platelets during blood clotting
44
Lithium Heparin tubes are used for
``` Glucose Blood Urea Nitrogen (BUN) Ionized Calcium Creatinine Electrolyte Studies ```
45
Lithium Heparin is not used for any test measuring...
lithium or folate levels
46
Sodium Heparin is not used for any test measuring
sodium concentration
47
Lithium Heparin with gel is used for...
plasma determinations in chemistry
48
Specific gravity
weight compared to water
49
Why use a lithium heparin with gel instead of a lithium heparin tube?
Because once centrifuged the gel forms a stable barrier separating plasma from the cells, allowing for easy collection of plasma
50
Lithium Heparin with gel inversion #
5-10 immediately
51
PST
Plasma Separator Tube
52
What test should Green tubes not be used for?
Blood smears that are to be stained with wright's stain as the Heparin causes blueish tint.
53
What tubes are best for assays that require heparinized plasma?
Mint green tubes because they have gel to separate the plasma from RBCs
54
Color used for most hematology procedures?
Lavender
55
Best tube for CBC?
Lavender
56
DIFF
Differentials
57
MCH
Mean Corpuscular Hemoglobin
58
MCHC
Mean Corpuscular Hemoglobin Concentration
59
MCV
Mean Cell Volume
60
K₂EDTA
Spray-Dried Potassium attached to EDTA
61
K₃EDTA
Liquid Potassium attached to EDTA
62
Which version of EDTA is better for hematology tests?
K₂EDTA
63
Which version of EDTA is better for viral markers?
K₃EDTA
64
Pink additive
K₂EDTA
65
Pink tubes are used for
blood bank collection
66
Pink inversion #
8-10
67
Tube that has label that meets AABB requirements
Pink
68
Gray additive
1) Potassium Oxalate and Sodium Fluoride 2) Na₂EDTA and Sodium Fluoride 3) Sodium Fluoride
69
Grey tubes are primarily used for
glycolytic inhibition testes
70
Sodium Fluoride prevents
glycolysis
71
glycolysis
breakdown or metabolism of glucose by blood cells
72
Grey Tube additives are also known as
Antiglycolytic agent and glycolytic inhibitor
73
Grey tubes should never be used for ____ and ____ determinations.
Sodium; Potassium
74
Why should grey tubes not be used for hematology studies?
Oxalate distorts cellular morphologic features
75
What can happen if a lavender tube is underfilled?
Falsely low blood counts Falsely low hematocrits Staining alterations on blood smears Erroneous morphologic changes to RBCs
76
Royal blue use
Trace element sudies
77
Tan tube additive
K₂EDTA
78
Black additive
Buffered Sodium Citrate
79
Black use
Determining ESR
80
ESR
Erythrocyte Sedimentation Rate
81
16 to 18 gauge needles are used for
Larger amounts of blood drawn
82
21 to 23 gauge needles are used for
Children and smaller specimen samples
83
Venipuncture Order of Draw (According to ETCH Card)
``` Blood Cultures Gold Light Blue Red Green Mint Green Grey ```
84
Which tube needs to go on ice?
Green with additive Ammonium Heparin
85
PTT
Partial Thromboplastin Time
86
Hct
Hematocrit