Chapter 8 - Transport in Plants and Transpiration Flashcards
Draw a cross-section of a plant root
Textbook page 135
Epidermis
(Root hair extensions should be present on the epidermal layer)
Cortex
Endodermis
Central stele (vascular cylinder) composed of vascular tissue (xylem and phloem)
Describe the structure of the endodermal layer in a plant root
Single layer of cells immediately outside the stele
Cells have a waterproof layer of suberin imbedded in their cell walls known as the Casparian strip
The layer of cells between the epidermis and endodermis is the …
Cortex
Cells in the cortex of a plant root are …
Undifferentiated
Have small air spaces between them
Are rich in starch grains
The stele is composed of …
Mainly xylem tissue
Smaller amounts of phloem tissue
Cambium tissue (meristematic region)
What are the two transport systems in flowering plants?
Xylem
Phloem
The xylem is differentiated into a number of cell types but the main type involved in water and ion transport is the …
Xylem vessel
How are xylem vessels specialised for the transport of water and inorganic mineral ions?
- No end walls
- No cell contents
- Dead when fully formed
- Have an impermeable secondary cell wall composed of lignin inside the primary cellulose cell wall
- Have pores
Why are mature xylem vessels dead?
Lignin is impermeable to water, so mature xylem vessels are dead.
What are the two types of xylem?
Protoxylem
Metaxylem
Why do xylem vessels have no end walls or cell contents?
No end walls = continuous tubes.
No cell contents = empty tube.
Therefore, water movement through xylem vessels requires less pressure than through living cells, where movement would be slowed down by cell contents.
As the xylem vessels form, their end walls …
Break down
Why do xylem vessels have a large vessel lumen?
Large empty lumen allows flow of large volumes of water
What is protoxylem and where is it found?
Protoxylem is first-formed xylem
Found in young growing regions (more specifically, the region of elongation) behind root and shoot tips
What are the four different types of patterns of lignification?
Spiral
Annular
Reticulate
Pitted
What is metaxylem and where is it found?
Mature (older) xylem
Found in more mature parts of the plant
What are the major differences between protoxylem and metaxylem?
- In metaxylem, the mature xylem vessels are dead.
- In metaxylem there is greater deposition of lignin (as the protoxylem cell walls are less thick, and due to the different patterns of lignification).
- Metaxylem is older compared to the younger protoxylem.
- Protoxylem cells are typically smaller.
What two patterns of lignification are present in protoxylem vessels?
Spiral
Annular
What two patterns of lignification are present in metaxylem vessels?
Reticulate
Pitted
Describe the pattern of thickening in spiral vessels of protoxylem
Cell wall thickening in the form of a continuous spiral.
Describe the pattern of thickening in annular vessels of protoxylem
Cell wall thickening in the form of discrete loops.
Why does lignin laid down in protoxylem vessels form annular and spiral patterns?
Lignin laid down in these patterns does not restrict the elongation of the xylem vessels along with other tissues as growth of root tips takes place
In the xylem found in the mature parts of the plant, known as metaxylem, there is greater …
Deposition of lignin
Describe the pattern of thickening in reticulate vessels of metaxylem
Reticulate vessels are thickened by interconnecting bars of lignin
Describe the pattern of thickening in pitted vessels of metaxylem
Pitted vessels are uniformly thickened, except at pores seen as pits that allow rapid movement of water and ions between adjacent vessels and surrounding cells.
What are the two properties of lignin?
- It provides great strength that prevents the vessels from collapsing when under pressure exerted by the transpiration stream ‘sucking’ water up the plant. This strength is also important in providing structural support for the plant.
- It is waterproof, which prevents the leakage of water.
A column of xylem vessels produces …
A long continuous tube up the plant
Draw all four patterns of lignification in xylem vessels
Textbook page 136
What is the function of the small pits present in pitted xylem vessels of metaxylem?
The small pits (pores) allow the movement of water and inorganic mineral ions between adjacent vessels and surrounding cells.
Knowledge check 16
List the features of xylem that make it well adapted to the transport of water.
Xylem vessels lack cross walls, lack any cytoplasm (to impede the flow of water), are lignified (to keep them open and prevent them collapsing) and have pores (to allow water to leave laterally).
Where are new xylem cells produced?
New xylem cells are produced in a meristematic region (the Cambium) between the xylem and the phloem in roots.
Where is protoxylem and metaxylem found in the xylem tissue of the stele of a plant root?
In roots the protoxylem is found on the outer edge of the stele with the metaxylem found innermost.
Reference the photograph of a section through a root vascular cylinder, textbook page 137
Draw a longitudinal and transverse section of metaxylem vessels
Textbook page 136
Longitudinal section
- Thick lignified walls
- Pits
- Cellulose cell wall of adjacent cells
- Remains of vessel cells’ end walls
- Xylem vessels
Transverse section
- Pit
- Vessel lumen
What are the two main cell types in phloem tissue?
Sieve tube elements
Phloem companion cells
The two transport systems in flowering plants (xylem and phloem) are contained within the plant’s …
Vascular tissues
Sieve tube elements are aligned end to end to form a continuous row of cells called …
The sieve tube
What is a sieve tube?
A continuous row of sieve tube cells (elements) aligned end to end.
What are the transporting cells in phloem tissue?
Phloem sieve tube cells (elements)
What are sieve plates?
Sieve plates are end walls in a sieve tube that consist of a thin cellulose cell wall that is perforated to form sieve pores.
How do xylem vessels and sieve tubes differ structurally?
Sieve tubes have end walls called sieve plates
Describe the ultrastructure of phloem sieve tube cells (elements)
Living cells with cell contents
No nuclei
Reduced volume of cytoplasm which is displaced to the side walls
Very few organelles are present
Microtubules extend between sieve elements and pass through the sieve pores
What is the role of microtubules present in sieve tube elements?
Microtubules extend between sieve elements and pass through the sieve pores. It is though that these are involved in the process of translocation.
Each sieve tube cell (element) is closely associated with one or more …
Phloem companion cells
Describe the ultrastructure of phloem companion cells
Dense cytoplasm rich in mitochondria and other organelles.
Have a high metabolic rate.
Companion cells are linked to the sieve tube elements by plasmodesmata.
Prominent nucleus.
What is the role of the plasmodesmata between each phloem sieve tube and its associated companion cell?
Plasmodesmata aid transport of sucrose into and out of sieve tube element
What is the function of the companion cells?
They act as supporting cells, carrying out many metabolic activities for the highly specialised sieve tube elements, allowing them to be specialised for the transport of organic solutes through the plant.
Why are the thin cell walls of phloem sieve tubes not lignified?
As the cells are not under tension
Why do sieve tube elements have their cytoplasm displaced to the side walls, few organelles and no nucleus?
Allows the movement of phloem sap through the centre of the cell (element)
Knowledge check 17
Explain the role of companion cells in translocation
Companion cells contain numerous mitochondria that produce the ATP used to carry out active processes such as loading sucrose etc. into the sieve tubes.
Draw a cross section of a plant stem
Textbook page 138
Epidermis Cortex Vascular bundles - Xylem (innermost) - Phloem (outermost)
Describe the distribution of vascular tissue in a plant stem
The vascular tissue is arranged as vascular bundles around the outside of the stem
Describe the distribution of vascular tissue in a plant root
Vascular tissue forms a central stele (vascular cylinder) in the root
In a plant stem, vascular tissue is arranged as vascular bundles around the outside of the stem. Why is this?
Provides greater support necessary in stems to support branches and leaves.
Where is protoxylem and metaxylem found in a plant stem?
In the vascular bundles, the xylem is found innermost (and the phloem is found outermost). Within the xylem, the protoxylem is usually in the section of xylem closer to the centre of the stem, with the metaxylem in the section of xylem closer to the outer edge of the stem.
Draw a cross section of a plant leaf showing the major tissue layers present
Textbook page 138
- Upper epidermis
- Midrib (xylem above phloem)
- Veins (xylem above phloem)
- Mesophyll
- Lower epidermis
As smaller branches (stems) continually branch they eventually form …
Leaves
As smaller branches (stems) continually branch they eventually form leaves. A vascular bundle continues into the leaf as the …
Midrib
As smaller branches (stems) continually branch they eventually form leaves. A vascular bundle continues into the leaf as the midrib, which branches to form smaller …
Veins that are distributed throughout the leaf
As smaller branches (stems) continually branch they eventually form leaves. A vascular bundle continues into the leaf as the midrib, which branches to form smaller veins that are distributed throughout the leaf. The leaf veins are typically found in …
The spongy mesophyll just below the palisade layer.
What is transpiration?
The evaporation of water from the cell surface membranes of the spongy mesophyll cells (and palisade mesophyll cells to some degree) into the intercellular air spaces and the subsequent diffusion of water vapour down the water potential gradient through the stomata (or through the waxy cuticle) and into the atmosphere.
It is convenient to consider the movement of water and ions through a plant as having three distinct phases:
- The transport of water (and ions) into and across the root.
- The transport of water up the root and stem in the xylem.
- The transport of water through the leaf and the evaporation of water from the leaf.
Knowledge check 15 Describe the distribution of: a) xylem b) phloem in both roots and stems
a) Xylem is the star-shaped tissue located centrally in the root and on the inner sides of the vascular bundles in the stem.
b) Phloem is found between the arms of the xylem in the root and on the outer sides of the vascular bundles in the stem.
What are the two types of transpiration?
Cuticular transpiration
Stomatal transpiration
What is the effective exchange surface in root hair cells?
The cell surface membrane
Water enters the root hair cells by …
Osmosis
Inorganic mineral ions enter the root hair cell by …
Active transport or facilitated diffusion (depending on the concentration gradient of the ion involved)
The cell surface membranes of root hair cells are …
Thin
Why does water enter the root hair cells by osmosis?
The water in the soil has a higher water potential compared to the root hair cell (which contains sugars and other compounds).
Once into the root hair cell, the water (and ions) move across the cells of the cortex and into the xylem in the stele. There are two main pathways involved:
- The apoplast pathway
* The symplast pathway
What is the apoplast pathway?
Water (and ions) move along the cellulose microfibrils of the cell walls.
What is the symplast pathway?
Water (and ions) move by osmosis from cell to cell through plasmodesmata.
Why and how does the apoplast pathway occur in the root?
The apoplast pathway - involves water (and ions) moving along the cellulose microfibrils of the cell walls.
As the water moves through the wall, the cohesive properties of the water (aided by hydrogen bonding) help pull the water column along.
Parallel arrangement of the microfibrils in the cellulose cell wall allows water to pass easily between the different layers, rather than through them.
The general mesh-like arrangement of the walls further aids movement.
Turn to textbook page 139 to see a diagram explaining this.
Most water tends to move through the cortex by the …
Apoplast pathway