chapter 8 soil micro Flashcards

(34 cards)

1
Q

soil layer and distribution of nutrients and microorganisms:

A

-layers: 1. topsoil 2. subsoil 3. bedrock
-organic nurtients: decrease woth depth
number of mos: decrease with depth but still present in bedrock

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2
Q

Environmental factors affecting microbial abundance in soils

A

Moisture content

Moist soils support microbial growth better than dry soils

Oxygen

Moist soils are lower in oxygen than dry soils

Oxygen dissolves poorly in water

pH

Highly acidic and highly basic soils favor fungi

Temperature

Most soil organisms are mesophiles

Nutrient availability

Microbial community size determined by how much organic
material is available
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3
Q

Based on their size, the organisms living in soil may be

categorized into 3 groups:

A

1.
Microbiota: viruses, bacteria, fungi, protozoa, algae
2.
Mesobiota (0.2 2mm): nematodes, mites, snails, …
3.
Macrobiota  2mm): rodents, earthworms, large
insects

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4
Q

Microbes perform a number of

functions

A


Cycle elements and convert them to usable form

Degrade dead organisms

Produce compounds with potential human uses

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5
Q

Characteristics of Soil Microorganisms

A

A
Bacteria:

Constitute the basic mass of all soil microorganisms

Most soil bacteria have the ability to adhere to surfaces of the
mineral molecules and to the soil colloids

Actinomycetes

Aerobic, Grow @40ºC 50 ºC

Degrade steroids, chitin, hydrocarbons, fatty acids

Many types produce antibiotics Erythromycin, Neomycin, Tetracycline,
Streptomycin

Rod coccus bacteria

2 60% of soil microflora

Highly resistant to environmental factors during the vegetative stage

Capable of surviving in dry soil for few months

They can utilize a wide spectrum of organic compounds as a food
substrate.

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6
Q

fungi

A


Eukaryotes, Heterotrophs

Grow strongly in acidic soils and have crucial influence on
changing of pH reaction

They get into symbiotic relationships with algae, insects, and
higher plants

They occur mostly in the upper layers of soil, however they
can be found as deep as 1 m

Most common: Penicillium , Aspergillus , Rhizopus

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7
Q

What habitats are provided by soil?

A


Naked rocks provide a very inhospitable habitat

These rocks can be colonized by Cyanobacteria that are
nitrogen fixating photolitotrophs : require only light and
inorganic nutrients to grow

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8
Q

Cyanobacteria

A

Cyanobacteria can provide both fixed nitrogen and
carbon compounds that can be used by other organisms

The action of Cyanobacteria initiates the biological
process that lead to soil formation and to nutrient cycling

The colonization of rocks by Cyanobacteria is the first
step in the transformation of naked rocks into soil suitable
for the support of plant and animal life

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9
Q

The microbes present in the soil are responsible for

A

The microbes present in the soil are responsible for re
cycling organic and inorganic material and play an
important part in the dynamic regeneration of soil

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10
Q

sandy soils

A

cannot retain water very well
and drain very quickly this may lead to the formation of
arid soils

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11
Q

clays

A

can retain water and also tend
not to be porous so as a result of water retention they also
tend to form anaerobic environments

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12
Q

Plants

A

are the major producers of organic material to befound in soil and plant matter accumulates as debris then
the animal feces and the decomposing bodies of dead
animals complement this organic supply

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13
Q

Artificially added fertilizers , herbicides and pesticides

all affect

A

the biological component and then the
organic contents of soil
-
Microbes play a central role in re cycling such
material and besides recycling of naturally occurring
organic compounds , soil microbes are responsible
for the chemical degradation of pesticides but not
all pesticides are easily broken down.

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14
Q

Biogeochemical cycles

A


Processes by which organisms convert elements from one form
to another

Elements often converted between oxidized and reduced
forms

Involve the recycling of elements by organisms

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15
Q

Biogeochemical cycling entails three processes

A

Production

Inorganic compounds converted into organic compounds

Consumption

Organisms feed on producers and other consumers

Decomposition

Organic compounds in dead organisms converted into
inorganic compounds
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16
Q

Photoautotrophs

A

such as cyanobacteria, green plants, algae,
and green and purple sulfur bacteria fix (incorporate) carbon
dioxide into organic matter using energy from sunlight

17
Q

Chemoautprotrophs

A

form also organic matter by using other

source of energy like H 2 S

18
Q

Chemoheterotrophs

A

such as animals and protozoa eat autotrophs
and may in turn be eaten by other animals.

Chemoheterotrophs , including animals, use some of the organic
molecules to satisfy their energy requirements.

When this energy is released through respiration, CO 2 immediately
becomes available to start the cycle over again

Much of the carbon remains within the organisms until they excrete
it as wastes or die.

19
Q

carbon cycle

A


When plants and animals die, these organic
compounds are decomposed by bacteria and fungi.

During decomposition, the organic compounds are
oxidized, and CO 2 is returned to the cycle.

Carbon is stored in rocks, such as limestone (CaCO 3 ),
and is dissolved as carbonate ions (CO 3
2 –) in

Vast deposits of fossil organic matter exist in the
form of fossil fuels, such as coal and petroleum.

Burning these fossil fuels releases CO 2 , increasing
the amount of CO 2 in the atmosphere  global
warming of the Earth

20
Q

Carbon Cycle

Methane (CH4) gas.

A


Sediments on the ocean floor contain an estimated 10 trillion tons of
methane (about twice as much as the Earth’s deposits of fossil fuels
such as coal and petroleum).

Furthermore, methanogenic bacteria in the ocean’s depths are
constantly producing more

Methane is much more potent as a greenhouse gas than is carbon
dioxide, and the Earth’s environment would be dangerously altered if
all this gas escaped to the atmosphere.

Fortunately, the majority of sea dwelling (home) bacteria use escaping
CH 4 gas as energy source so it disappears before reaching the surface of
the water

21
Q

Nitrogen Cycle

A


Nitrogen is needed by all organisms for the synthesis of proteins,
nucleic acids, and other compounds

Molecular Nitrogen N 2 makes up about 80 % of the Earth’s
atmosphere

For assimilation and use by plants nitrogen must be fixed and
transformed to organic compounds

The activities of specific microorganisms are important to the
conversion of nitrogen to usable forms

22
Q

A

Ammonification

A


When an organism dies, the process of microbial decomposition
results in the hydrolytic breakdown of Proteins into amino acids

23
Q

Deamination :Ammonification

A


Deamination : the amino groups of amino acids are removed and
converted into ammonia (NH 3

This release of ammonia is called Ammonification which can be
carried by numerous bacteria and fungi

24
Q

ammonification in soils

A
1
Dry Soil: Ammonia rapidly disappears
2
Moist soil: it becomes solubilized in water, and ammonium ions (NH 4 ++) are
formed:
N.B.
Ammonium ions from this sequence of reactions are used by bacteria and
plants for amino acid synthesis.
25
Nitrification :
the oxidation of the nitrogen in the ammonium ion to produce nitrate - Plants tend to use nitrate as their source of nitrogen for Protein synthesis because nitrate is highly mobile in soil  NH4+ would actually make a more efficient source of nitrogen because they require less energy to incorporate into proteins  These positively charged ions are usually bound to negatively charged clays in the soil, whereas the negatively charged nitrate ions are not bound
26
C | Denitrification
 The form of nitrogen resulting from nitrification is fully oxidized and no longer contains any biologically usable energy  However, it can be used as an electron acceptor by microbes metabolizing other organic energy sources in the absence of atmospheric Oxygen  Denitrification can lead to a loss of N 2 to the atmosphere especially as N 2 Gas NO 3  NO 2  N 2 O (Nitrous oxide)  N 2  Pseudomonas appear to be the most important group of bacteria in denitrification in soils that occurs in water logged soils where little O 2 is available
27
D | Nitrogen fixation
 The air we breathe is about 79 80 % of Nitrogen  Few bacterial species including Cyanobacteria can use atmospheric Nitrogen directly as nitrogen source  Nitrogen fixation: The process by which bacteria convert nitrogen gas to ammonia ( Nitrogenase
28
 Nitrogen fixation is brought about by two types of microorganisms: free living and symbiotic.
 Free Living Nitrogen Fixing bacteria are found in the rhizosphere (region roughly 2 mm from the plant root). Ex: A zotobacter , Clostridium pasteurianum  Symbiotic Nitrogen Fixing Bacteria: play an even more important role in plant growth for crop production. Ex: members of the genera Rhizobium, Bradyrhizobium P.S. Agricultural fertilizers are made up of nitrogen that has been fixed by industrial physical chemical processes
29
Sulfur Cycle
H 2 S represents a source of energy for autotrophic bacteria  These bacteria convert the reduced sulfur in H 2 S into elemental sulfur granules and fully oxidized sulfates (SO 4 2  Several phototrophic bacteria, such as the green and purple sulfur bacteria also oxidize H 2 S  Hydrogen sulfide can be used as an energy source by Thiobacillus to produce sulfate ions & sulfuric acid.  Plants and certain microbes can use SO 4 2 to make amino acids  As proteins are decomposed, in a process called dissimilation, the sulfur is released as hydrogen sulfide to reenter the cycle
30
Sulfur Cycle (cont’d)
 Burning fossils fuels that contain sulfur from the remainings of dead organisms release sulfur in the form of SO 2 (sulfur dioxide ) Natural phenomena like volcanic eruption also release large amounts of SO 2  This SO 2 will react with H 2 O to form H 2 SO 3 the acid rain  Acid deposition often impairs the growth of trees, reacts with metals, marble, ….  The acidification of lakes and streams affect the pH of water to levels that are very acid to forbid the growth of fish or essential units of their food chain  Moreover, NO 2 (nitrogen oxide) from vehicles, a large component of urban smog enter waters and will be transformed to acid
31
Phosphorus Cycle
 Environmental phosphorus undergoes little change in oxidation state  Phosphorus converted from insoluble to soluble forms , becomes available for uptake by organisms  Conversion of phosphorus from organic to inorganic forms Occurs by pH dependent processes
32
Samples Collection
As soils have discontinuous + heterogeneous (MO )= necessity to obtain a representative sample • As such, sampling strategy should be designed to ensure a quality assurance plan
33
Soils samples collection (cont’d) | In general a quality assurance plan include the followings
 Sampling strategies (number type of samples locations, depths)  Sampling methods (specific techniques + needed equipment)  Sample storage (type of containers+ preservation methods + holding times)
34
I Sampling strategies
 Soil samples can be obtained by shovel or auger (more precise and can be used to depths up to 180 cm)  Several samples should be collected from a site to have a representative sample  It’s better to analyze all samples b ut due to time and financial resources a composite sample can be done