Chapter 8 - Analyzing Cells, Molecules and Systems

Question 1

Genome engineering

Answer 1

Also known as genome editing

Genome of organisms is deliberately altered in a particular way

Question 2

Gene knockouts

Answer 2

Removal of a gene (including all copies) in order to discover the function of that gene

Very useful if gene is not essential

Question 3

Reverse genetics technique

Answer 3

1. Gene knockout
2. Expressing gene in wrong tissue or at the wrong time - provides important clues of genes normal function
3. Expressed normally in most cells but deleted in some cells - useful when a gene has different roles in different tissues

Question 4

Reverse genetics

Answer 4

Start with a gene, mutate it to analyze the genes function