Chapter 6: Genetic Stratagies in Therapheutics

Question 1

Give 6 examples of the Protein Products of Recombinant DNA Technology

Answer 1

Insulin, Growth factor, Epidermal growth factor(Burns, ulcers), Hepatitis B vaccine(Prevention of viral hepatitis), Erythropoietin(Anemia), Factor VII(Hemophilia)

Question 2

Explain gene replacement therapy for cystic fibrosis.

Answer 2

Bacterial cloning of human therapeutic gene, cloned therapeutic transgene inserted into Adeno-assiciated virus. AAV containing therapeutic transgene nasally dripped into lung, which enters lung cell nucleus. Trerapeutic transgene corrects genetic defect.

Question 3

What is Ex vivo gene replacement therapy, and tell me some disease using this cure

Answer 3

Ex vivo gene replacement therapy involes the genetic manipulation of a patient’s target cells outside the body. Modified cells are then reintroduced into the same patient. Ex. X-linked Severe Combinded Immunodeficiency.