Chapter 6 - A Tour of the Cell Flashcards
larger organisms like plants and animals are…
multicellular
when were microscopes invented?
1590 (and were further refined during 1600s)
who was the first to see cell walls, when, and where?
Robert Hooke in 1665 - through a microscope while examining dead cells from the bark of an oak tree
who was the first to visualize living cells?
Antoni van Leeuwenhoek roughly 1674
what are light microscopes and how do they work?
light is passed through a specimen and through glass lenses -> lenses refract/bend the light in such a way that the specimen image is magnified as it’s projected into the eye or into a camera
what are three important parameters in microscopy?
magnification
resolution
contrast
what is magnification?
the ratio of an object’s image size to its real size
how much can light microscopes magnify?
about 1,000x the actual size of a specimen
what is resolution?
a measure of the clarity of the image; the minimum distance two points can be separated and still be distinguished as separate points
a light microscope cannot resolve detail finer than…
0.2 micrometers, or 200 nanometers - regardless of the magnification
a Paramecium is considered a…
single-celled organism
define organelles
membrane-enclosed structures within eukaryotic cells
when was the electron microscope introduced?
1950s
how does an electron microscope work?
a beam of electrons passes through a specimen or onto its surface
resolution is inversely related to the wavelength of the light a microscope uses for imaging
what resolution can electron microscopes achieve?
0.002 nm; cannot resolve structures smaller than 2 nm across
this is still a 100-fold improvement over the standard light microscope