Chapter 4-6 Flashcards
Functions of cerebrospinal fluid?
- cushions brain
- removes waste
- supports neurogenesis
- contains immune-related molecules
- controls neurotransmitter precursors
What structures comprise the brain stem?
medulla, tectum, tegmentum, pons
Describe how the blood-brain barrier is different in the medulla
In the area postrema, the blood-brain barrier is more porous than the rest of the brain
Describe the effects of cerebellar agensis
Only mild/moderate decifits in balance and motor control
What are the four cranial nerves in the medulla?
Glossopharyngeal, vagus, accessory, hypoglossal
Describe how Wilder Penfield provided evidence for localization of function?
By stimulating the somatosensory and motor cortexes while the patient was awake he was able to map parts of the body onto the brain. The amount of tissue associated with parts of the body was proportionate to the amount of sensation experienced in that body part
Name the neurotransmitter systems and what processes they are involved in
Cholinergic - learning, memory, arousal
Dopaminergic - motor control, reward processing, motivation, addition
Noradrenergic - arousal, sustained attention
Serotonergic - arousal, mood, sleep
Describe the preparation process of the sample in visible light transmission microscopy
- sample is fixed in place using formaldehyde
- sample is dehydrated using acetone
- sample is hardened using paraffin
- sample is sliced into very thin pieces using a microtome
- certain structures are stained to observe them more closely
Describe the two types of electron microscopy
TEM - electrons are fired through the sample and the ones that make it through strike a phosphorus plate and the others bounce back. Heavy metals are used to stain the sample which decreases the number of electrons that can pass through
SEM - electrons are moved back and forth across sample to see how many are backscattered
Advantages and disadvantages of CAT scan
A - can be used to create 3D reconstruction, don’t take very long
D - requires radiation, requires contrast dyes
Advantages and disadvantages of MRI
A - no radiation, no contrast dyes
D - takes a long time, expensive
Describe how Diffusion Tensor Imaging (DTI) works and its purpose
Tracks the movement of water through the 3 axon bundles in the brain (corona radiata, cingulum, inferior longitudinal faciculus). Used to determine anatomical connectivity between structures across the brain
Describe how fMRI uses blood flow to measure structural changes in brain
Brain structures that are more active require more O2 from blood and so blood vessels in the capillaries in these regions dilate to allow more deoxyhemoglobin to pass into brain. This process decreases the amount of deoxyhemoglobin which increases the MRI signal.
Describe why PET uses glucose
Because glucose is the brain’s main source of energy for neural activity, PET involves injecting version of glucose to see where it is used in the brain. This will show where brain activity is occuring.
Describe why fNIRS is more ideal than PET or fMRI
Because it is less expensive and portable
Describe which imaging techniques measure brain activity indirectly vs directly
fMRI, PET, fNIRS vs EEG, MEG
List 3 ways proteins can be introduced to the body in optogenetics
Transgenic animals, viral delivery, gene gun
Describe one practical application of tDCS
Helping stroke victims regain function of their limbs on their affected side
Name one disorder that EBS may be used to treat
Major Depression
What are all the ways optogenetics can be used?
At the synapse, neuron, local circuit, intralayer, whole brain, behaviour
Which imaging technique can be used to create a “virtual patient”?
TMS/rTMS