Chapter 3 Proteases

Question 1

Trypsin

Answer 1

Lysine, Arginine @ C

Question 2

Pepsin

Answer 2

Leucine, Phenylalanine, Tyrosine, Tryptophan @ N

Question 3

Staphylococcus aureas V8

Answer 3

Aspartate, Glutamate @ C

Question 4

Asp - N - protease

Answer 4

Aspartate, Glutamate @ N

Question 5

Dansyl/Dabsyl Chloride

Answer 5

N-terminal identifier

Question 6

DTT or Clealand’s Reagent

Answer 6

Reduction of S-S bonds

Question 7

FDNB

Answer 7

N-terminal identifier

Question 8

Endoproteinase Lys-C

Answer 8

Lysine @ C

Question 9

Hydrazinolysis

Answer 9

C-terminal identification

Question 10

Performic acid

Answer 10

Oxidation of S-S bonds

Question 11

Chymotripsin

Answer 11

Phenylalanine, Tyrosine, Tryptophan @ C

Question 12

PITC or Edmand’s Reagent

Answer 12

N-terminal identifier

Question 13

Cyanogen Bromide

Answer 13

Methionine @ C

Question 14

Submaxillarus protease

Answer 14

Arginine @ C

Question 15

Carboxypeptidases

Answer 15

C-terminal identification